TFome pUT6728
Template RT-PCR
Genbank Insert ID KJ728423
Protein Name ZmOrphan294
Species Zea mays
TF Family Orphans
Gene id GRMZM2G170299
transcript T01
Vector pENTR/SD/D-TOPO
Note c.C291T p.S97(=)
Request Information Deposited to ABRC
Subgenome: 1

TFome clone pUT6728

This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.

5' Primer

Name
Temperature (ºC)
Sequence
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3' Primer

Name
Temperature (ºC)
Sequence
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PCR Condition

Amino Acid Sequence
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MGSCLSSEAPAAGDGPAAWRKRRHGSREGAAAGAGAAGGGGKKLPGGAGEMTEDELARVPGRLCGKGASAVACLHTQQGRKGTNQDAMVVWESFNSSDSIFCGVFDGHGPYGHFVAKKVRDSLPVKLRTQWQTSANGGSSPHQNGSINSEETGSIVDDEWGDGDDTEKLPEMFLPLKQSYLKAFKLMDKELKLHPTVDCFCSGSTAVTLVKQGLDLVIGNLGDSRAIMGTRDASNNLTALQLTVDLKPNLPREAARIQQCKGRVFALQDEPEVARVWLPNNDSPGLAMARAFGDFCLKDYGLISVPEISYRRLTEKDEFIILATDGVWDVLSNKEAVDIVASAPSRATAARALVDCAVRSWRLKFPTSKSDDCAVVCLFLGHEKSADSVQGSEPNVETAEPTVEVSSSTQDSSAGVDEDIADAGMHVSSAVHISEATLQHSTTLREVDEIVPVDEPTASKEPGRCGSARSLADCISTNEEEEWSALEGVTRVNSLLNLPRLLSGDKRSTSWRKRR

TFome clones do not contain stop codon at 3'end.

CDS
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ATGGGCTCCTGCCTCTCCTCCGAGGCCCCCGCCGCCGGCGATGGCCCAGCGGCGTGGCGGAAGCGGCGCCACGGGAGCCGGGAGGGCGCGGCCGCCGGCGCCGGCGCCGCCGGCGGCGGTGGGAAGAAGCTGCCCGGAGGAGCGGGGGAGATGACGGAGGACGAGCTCGCGCGGGTCCCCGGGAGGTTGTGCGGCAAAGGCGCCAGCGCGGTGGCGTGCCTCCACACGCAGCAGGGCCGGAAGGGCACCAACCAGGACGCCATGGTCGTGTGGGAGAGTTTTAATTCAAGTGACAGCATCTTCTGTGGTGTGTTTGATGGCCATGGGCCATACGGTCATTTTGTTGCAAAGAAAGTTAGAGATTCACTTCCTGTCAAGTTGCGCACACAATGGCAAACTAGTGCTAATGGGGGCTCTAGTCCTCATCAAAATGGAAGCATCAACTCCGAAGAAACAGGGTCTATTGTTGACGATGAATGGGGTGATGGTGATGACACTGAAAAGCTCCCTGAGATGTTTCTTCCACTGAAGCAATCTTACCTAAAAGCTTTCAAATTGATGGACAAGGAACTCAAGCTGCATCCCACAGTTGACTGTTTCTGCAGTGGATCTACGGCAGTAACATTGGTGAAACAGGGATTGGACCTTGTAATCGGAAATCTTGGGGACTCAAGAGCAATTATGGGGACAAGAGACGCCTCTAACAATCTAACTGCTCTGCAACTCACAGTTGATTTGAAGCCTAACCTTCCCAGGGAAGCTGCGAGGATACAACAATGTAAAGGACGGGTCTTTGCTCTTCAGGATGAGCCAGAAGTTGCCAGGGTATGGTTGCCAAATAATGACTCTCCTGGATTGGCAATGGCAAGAGCCTTCGGGGACTTCTGCCTTAAAGATTATGGTTTAATATCTGTTCCAGAGATATCATATCGCCGCCTTACCGAAAAGGATGAATTTATAATACTAGCCACCGATGGGGTATGGGATGTTCTCTCAAATAAGGAGGCTGTCGACATAGTAGCATCAGCCCCATCTCGTGCGACAGCAGCCAGGGCCCTTGTTGACTGTGCTGTCAGGTCATGGAGGTTGAAGTTCCCAACATCCAAGAGCGATGACTGTGCTGTTGTCTGCTTATTCTTGGGTCACGAGAAGTCGGCCGACTCGGTTCAAGGGAGTGAACCCAATGTGGAGACAGCAGAACCCACCGTGGAGGTTTCCAGTTCCACACAGGATTCCAGTGCAGGGGTTGACGAAGACATTGCAGATGCTGGCATGCATGTATCTAGTGCAGTGCACATTTCCGAGGCCACGCTGCAGCACTCCACCACGTTAAGGGAGGTCGACGAGATTGTACCGGTCGACGAACCTACAGCCTCGAAGGAGCCTGGAAGGTGCGGGTCTGCTCGCAGCCTGGCAGATTGCATATCGACAAACGAGGAAGAGGAATGGTCCGCGCTCGAAGGCGTGACACGGGTGAATTCCCTCCTGAACCTCCCTAGACTACTGTCGGGCGACAAGCGATCGACCAGCTGGAGGAAGCGGCGG


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