| TFome pUT1136 | |
|---|---|
| Template | EE188777 |
| Genbank Insert ID | HQ858680 |
| Protein Name | ZmDOF1 |
| Species | Zea mays |
| TF Family | C2C2-Dof |
| Gene id | AC155434.2_FG006 |
| transcript | T006 |
| Vector | pENTR/D-TOPO |
| Note | c.Start+111 Present in FlcDNA. |
| Request Information | Deposited to ABRC |
TFome clone pUT1136
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | C16 UP |
| Temperature (ºC) | 65.3 |
| Sequence
|
CAC CAT GGA GCT CGC CGG AGC |
3' Primer
| Name | C16 DN |
| Temperature (ºC) | |
| Sequence
|
CGTCGTCTCCTGAAAAGActgc |
PCR Condition
Step 1, 98C 30 sec melt, 1 cycle; Step 2, 98C 10 s
Amino Acid Sequence
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MELAGAASPHPPPESHVAPPRPPPQPPEEDLCEDRGDMSVTGEKPCTHRESDVGQTNSCSLNNSSECENHTPSNDEISEPESNLEMAKTDQGGDVPSGEKVLKKPDKILPCPRCNSMDTKFCYYNNYNIKQPRHFCKSCQRYWTAGGSMRNIPVGAGRRKSKSSSASCRSVLIPVPGSSSVANPGGEASLFPLSVKANQAAVSFGPDSPLCTSMASVLKIGGGGEQVKSCSPASAAAQPRNGETQTQTCAPSSAATTPSDGPGNGLQKGAESAHQNQNQNQNGIIGHSNGVTPVHPIPFFPGPPFVYPWSPAWNGIPAMAAAVCAAPATAEAAISSEHGTASSTVQWNVPPAIVPVLPPGFCGPIPVPVIPPPSVWPLITPWPNAAWSAPWLGPSASVPPGSSRSSGSSTCSDSGCGSGSPVLGKHSRESRPQGDEKAERRCLWIPKTLRIDDPVEAAKSSIWTTLGIEPGDRGMFRPFQSKHGRQQELQASGAARALQANPAALSRSQSFQETT
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGAGCTCGCCGGAGCAGCCTCCCCTCACCCGCCGCCGGAATCCCACGTGGCGCCGCCCCGCCCACCTCCGCAGCCGCCGGAAGAGGATTTATGTGAAGATAGAGGGGACATGAGTGTCACCGGCGAAAAGCCATGCACACATCGGGAATCAGATGTTGGTCAGACAAATAGTTGTAGCCTTAACAATTCCAGTGAGTGTGAGAATCATACACCCAGCAACGACGAAATATCGGAACCAGAGTCCAATTTGGAGATGGCCAAGACTGATCAGGGCGGCGATGTGCCGAGCGGAGAGAAGGTCCTGAAGAAGCCAGACAAGATCCTGCCGTGCCCTCGCTGCAACAGCATGGACACGAAGTTCTGCTACTACAACAACTACAACATCAAGCAGCCAAGGCATTTCTGCAAGAGCTGTCAGAGGTACTGGACCGCAGGCGGAAGCATGAGAAACATCCCCGTTGGTGCTGGGAGGCGCAAGAGCAAGAGCTCCAGCGCGAGTTGCCGCAGCGTGTTGATTCCCGTTCCAGGCAGCAGTAGTGTAGCTAATCCTGGCGGAGAGGCTTCCCTGTTTCCGTTGTCCGTAAAAGCAAACCAAGCAGCAGTTAGCTTCGGGCCTGATTCCCCCCTCTGCACCTCCATGGCCTCCGTGCTGAAGATCGGAGGAGGAGGAGAGCAGGTTAAGAGCTGCAGCCCTGCCTCAGCAGCAGCACAGCCCAGGAACGGAGAAACCCAGACCCAGACGTGCGCACCTTCTTCTGCTGCTACAACACCATCAGATGGTCCAGGGAATGGATTGCAGAAAGGAGCAGAGAGCGCACACCAAAACCAAAACCAAAACCAAAACGGAATCATTGGGCACAGCAACGGAGTCACTCCAGTGCATCCTATACCGTTCTTCCCCGGACCGCCTTTCGTGTACCCCTGGAGTCCAGCATGGAACGGCATTCCCGCCATGGCAGCGGCGGTGTGCGCAGCCCCAGCGACAGCCGAAGCAGCGATTTCATCAGAACACGGCACCGCGAGCAGCACCGTCCAGTGGAACGTGCCACCAGCGATCGTGCCCGTGCTGCCACCGGGATTCTGCGGCCCGATCCCAGTCCCGGTAATCCCGCCGCCTTCCGTCTGGCCACTGATCACTCCCTGGCCCAACGCAGCATGGAGCGCGCCGTGGCTCGGGCCTAGCGCTAGCGTGCCGCCGGGGTCATCTCGGAGCAGCGGCAGCAGCACGTGCTCCGACAGCGGCTGCGGCTCCGGCTCCCCCGTCCTGGGAAAGCACTCGAGGGAGTCGAGGCCGCAGGGCGACGAGAAGGCGGAACGACGGTGCCTGTGGATCCCCAAGACGCTCCGGATCGACGACCCTGTCGAGGCCGCCAAGAGCTCGATCTGGACGACGCTCGGGATCGAGCCTGGCGACCGGGGCATGTTCAGGCCGTTCCAGTCGAAGCATGGACGGCAGCAGGAGCTGCAAGCGTCCGGCGCCGCTCGCGCCCTGCAGGCCAACCCGGCGGCTCTGTCGCGCTCGCAGTCTTTTCAGGAGACGACG
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