| TFome pUT1139 | |
|---|---|
| Template | EE177657 |
| Genbank Insert ID | HQ858683 |
| Protein Name | ZmEREB2 |
| Species | Zea mays |
| TF Family | AP2/ERF-AP2 |
| Gene id | GRMZM2G080516 |
| transcript | T01 |
| Vector | pENTR/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
TFome clone pUT1139
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | C22 UP |
| Temperature (ºC) | 66.0 |
| Sequence
|
caccATGCTGCTGAACCCGGCG |
3' Primer
| Name | C22 DN |
| Temperature (ºC) | |
| Sequence
|
GACGACGATCTGCTCCACgc |
PCR Condition
Step 1, 98C 30 sec melt, 1 cycle; Step 2, 98C 10 s
Amino Acid Sequence
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MLLNPASEASVLDTIRQHLLEEPADESFGSLVADQWSGSLPFRTDDADDMVVFGALQDAFAYGWLPDGSFVHVKPEPVRSPDSSSYPCSYDGSPCFGLLDPEPPLTPGTTTPSSRGQEEAAAAMARGKHYRGVRQRPWGKFAAEIRDPARNGARVWLGTYDTAEDAALAYDRAAYRMRGSRALLNFPLRIGSGDKRPSPAPPEPATSSDSSSSSASGSHKRRKRGEAAAANMAMALVPPPSQLNRPAQPWFPAAPVEQAAMAPRVEQIVV
TFome clones do not contain stop codon at 3'end.
CDS
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ATGCTGCTGAACCCGGCGTCAGAGGCGTCGGTGCTAGACACCATCCGGCAGCACCTCCTCGAGGAGCCAGCCGACGAGAGCTTCGGGAGCCTGGTGGCGGACCAGTGGAGCGGCTCGCTCCCGTTCCGCACCGACGACGCCGACGACATGGTGGTGTTCGGGGCGCTGCAGGACGCCTTCGCCTACGGCTGGCTGCCCGACGGCTCATTCGTGCACGTGAAGCCCGAGCCGGTGCGGTCCCCCGACTCGTCCTCCTACCCCTGCTCCTACGACGGCTCACCCTGCTTCGGCCTCCTGGACCCGGAGCCGCCGCTGACGCCCGGCACCACCACGCCCAGTAGTAGGGGGCAGGAGGAGGCCGCGGCGGCCATGGCCCGGGGCAAGCACTACAGGGGGGTGAGGCAGCGCCCGTGGGGCAAGTTCGCGGCGGAGATCAGGGACCCCGCCAGGAACGGCGCGCGCGTCTGGCTCGGCACGTACGACACCGCCGAGGACGCCGCGCTCGCCTACGACCGCGCCGCCTACCGCATGCGCGGCTCGCGCGCGCTCCTCAACTTCCCGCTCCGCATCGGCTCCGGGGACAAGCGCCCGTCGCCGGCGCCGCCCGAGCCCGCCACCTCCTCGGACTCCTCCTCGTCTTCGGCCAGCGGCTCGCACAAGAGGCGGAAGCGAGGCGAGGCCGCGGCTGCCAACATGGCCATGGCGCTGGTGCCCCCGCCCTCCCAGCTTAACCGGCCGGCCCAGCCGTGGTTCCCTGCCGCGCCGGTCGAGCAGGCGGCGATGGCTCCGCGCGTGGAGCAGATCGTCGTC
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