TFome clone pUT1141

This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.

5' Primer

Name	C26 UP
Temperature (ºC)	69.8
Sequence Copy to clipboard	CAC CAT GAC GCC GGC GGC GTC G

3' Primer

Name	C26 DN
Temperature (ºC)
Sequence Copy to clipboard	GCTAATCGCCGCCTCCCG

PCR Condition

Step 1, 98C 30 sec melt, 1 cycle; Step 2, 98C 10 s

Amino Acid Sequence

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MTPAASPAQFAPAPLRMGYGRPAPVMGMWSSEPFKVDSGGHATCSASTVMEADTKLETSRLQDVPQVALEPERSTDQETSRPPERVMRRLAQNREAARKSRLRKKAYIQQLETSRMKLAQLELELQRARRQQGAYANGSMGDPALGYTGSIDPGVAAFEIEYRHWVDEQKRHTAELMSALQGQQTSELELRLLVETGLSNYEHLFRIKALAANADVFHVMSGVWKTPAERFFLWIGGFRPSEVLKILSPQLEPLAEAQRMLVGGLQHTSTQAEDALSQGMEKLQQNLAEILTAEADPFGAPDAYMLQMATAVEKLKELVNFVTQADHLRLMTLQQMHKILTTRQAARGLLALGDYFQRLRTLSSLWAARPREAAIS

TFome clones do not contain stop codon at 3'end.

CDS

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ATGACGCCGGCGGCGTCGCCCGCGCAGTTCGCGCCGGCGCCCCTCAGGATGGGGTACGGGCGGCCGGCGCCCGTGATGGGCATGTGGAGCAGCGAGCCGTTCAAGGTCGACAGCGGCGGGCACGCCACCTGCAGCGCGTCCACCGTCATGGAGGCGGACACCAAGCTCGAGACAAGCAGGCTACAAGATGTTCCACAGGTAGCACTCGAGCCAGAGAGGAGTACGGATCAGGAAACGAGCAGGCCGCCAGAGAGGGTCATGAGAAGGCTCGCGCAGAACAGGGAGGCTGCTCGGAAGAGTCGCCTGCGGAAAAAGGCCTACATCCAGCAGCTAGAGACAAGCCGGATGAAGCTGGCCCAGCTAGAGCTGGAGCTTCAACGGGCTCGACGGCAGCAGGGCGCGTACGCAAATGGGAGCATGGGAGACCCAGCTCTCGGATACACAGGGTCGATCGATCCAGGCGTCGCCGCGTTCGAGATCGAATACAGGCACTGGGTGGACGAGCAGAAGCGGCACACGGCGGAGCTGATGTCGGCGCTCCAGGGGCAGCAGACGTCGGAGCTGGAGCTCCGCTTGCTGGTGGAGACGGGGCTCAGCAACTACGAGCACCTCTTCAGGATCAAGGCGCTGGCCGCCAACGCGGACGTGTTCCACGTCATGTCGGGCGTGTGGAAGACCCCCGCCGAGAGGTTCTTCCTCTGGATCGGCGGGTTCAGGCCGTCGGAGGTCCTAAAGATTCTGAGCCCGCAGCTGGAGCCGCTGGCGGAGGCGCAGCGCATGCTCGTGGGCGGCCTCCAGCACACGTCAACGCAGGCGGAGGACGCGCTGTCGCAGGGCATGGAGAAGCTACAGCAGAACCTCGCCGAGATCCTGACCGCCGAGGCTGACCCCTTCGGGGCCCCCGACGCCTACATGCTGCAGATGGCCACTGCGGTGGAGAAGCTGAAAGAGCTCGTCAACTTCGTCACCCAGGCGGACCATCTCCGGCTGATGACGCTGCAACAGATGCACAAGATCCTGACGACGCGGCAGGCGGCCAGGGGCCTGCTGGCGCTCGGCGACTACTTCCAGCGCCTCCGCACGCTGAGCTCGCTGTGGGCGGCGCGCCCGCGGGAGGCGGCGATTAGC