TFome clone pUT1145

This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.

5' Primer

Name	C37 UP
Temperature (ºC)	65.0
Sequence Copy to clipboard	CAC CAT GGC CGC CGG AGC

3' Primer

Name	C37 DN
Temperature (ºC)
Sequence Copy to clipboard	AGCGTATCTACAGTCGTGAagc

PCR Condition

Step 1, 98C 30 sec melt, 1 cycle; Step 2, 98C 10 s

Amino Acid Sequence

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MAAGAGAGDEEKDDAVLPGFRFHPTDEELVTFYLRRKVARKPLSMEIIKEMDIYKHDPWDLPKASTAAGGEKEWYFFCLRGRKYRNSIRPNRVTGSGFWKATGIDRHIYPATANPVESVSVGLKKSLVYYRGSAGKGTKTDWMMHEFRLPPAAASPSPTPTSMQEAEVWTICRIFKRNIAYKRQPQLPAWRQQQQSSSNTGSFESDGGGGGDEYMNRCLPVPATATATAHQIGGMLNGGGVVSATGSSSSSFFRDRESVHGGQQFHFHEQWLSRFPAAPAVEQKPQLLDPSAMAIAFHQNDDQSVATAAAAAASECCKDGYYWDEIARFMEVNDPTVLHDCRYA

TFome clones do not contain stop codon at 3'end.

CDS

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ATGGCCGCCGGAGCCGGAGCCGGAGATGAAGAGAAAGATGACGCGGTGCTCCCTGGGTTCCGGTTCCACCCGACCGACGAGGAGCTCGTCACGTTCTACCTCCGCCGGAAGGTGGCCAGGAAGCCGCTCAGCATGGAGATCATCAAGGAGATGGACATCTACAAGCATGATCCATGGGACCTCCCTAAGGCGAGCACGGCTGCTGGTGGCGAAAAGGAGTGGTACTTCTTCTGCCTGAGAGGAAGGAAGTACCGGAACAGCATCAGGCCCAACAGGGTCACCGGCTCCGGCTTCTGGAAGGCCACCGGCATCGACCGGCATATCTACCCTGCCACCGCGAACCCCGTCGAGTCCGTGTCCGTCGGGCTCAAGAAGTCCCTCGTGTACTACCGCGGCAGCGCCGGCAAGGGCACCAAGACCGACTGGATGATGCACGAGTTCCGCCTCCCGCCGGCCGCCGCCTCGCCCTCGCCAACGCCGACGAGCATGCAAGAAGCTGAGGTGTGGACCATCTGCAGGATCTTCAAGAGGAACATCGCCTACAAGAGGCAGCCGCAGCTGCCGGCGTGGAGGCAGCAGCAGCAGTCCAGCTCCAACACGGGGAGCTTCGAGTCCGACGGCGGCGGCGGCGGGGACGAGTACATGAACCGCTGCCTGCCGGTGCCGGCCACGGCCACGGCCACCGCTCATCAGATCGGCGGCATGCTGAACGGAGGAGGCGTCGTCAGTGCCACTGGCAGCAGCAGCAGCAGTTTCTTCAGGGACAGGGAGAGCGTGCACGGCGGCCAGCAATTCCACTTCCACGAGCAGTGGCTGAGCCGCTTCCCCGCCGCGCCAGCAGTCGAGCAGAAGCCGCAGCTCCTGGACCCGTCGGCGATGGCCATCGCGTTCCATCAGAACGACGACCAGAGCGTCGCCACCGCTGCCGCTGCCGCTGCGAGCGAGTGCTGCAAGGACGGGTACTACTGGGACGAGATCGCGAGGTTCATGGAGGTCAACGATCCAACGGTGCTTCACGACTGTAGATACGCT