TFome clone pUT1147

This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.

5' Primer

Name	D1 UP
Temperature (ºC)	63.0
Sequence Copy to clipboard	CAC CAT GGG GAG GTC CCC G

3' Primer

Name	D1 DN
Temperature (ºC)
Sequence Copy to clipboard	TAGTGGCAGGCTGAAGCTgc

PCR Condition

Step 1, 98C 30 sec melt, 1 cycle; Step 2, 98C 10 s

Amino Acid Sequence

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MGRSPCCCHDAGVKKGPWTEEEDRALVDHIQRHGGHVSSWRNLPKAAGLNRCGKSCRLRWTNYLRPDIKRGNFTDDEERLIIGLHAQLGNRWSTIATHLDGRTDNEIKNYWNTHIRKKLLRMGVDPVTHQRLPPDDLLLAVSASPTTTILSAAASQLRGLNTALMQVQALQFLLQAVSGVGNDVNAAAAAANGGGGLVPSLSAAADNNAVLNASRSVVPGFQDLQLTTMTRPLLVSHANHGSADHHPSYIATDDVVQRRLNASAPPVVGGGACAEPLAVALASTAYRHQVAAAADEPVNGFAELILSEPIEMPSMCSLEDEDHFWKDMLVESSSFSLPL

TFome clones do not contain stop codon at 3'end.

CDS

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ATGGGGAGGTCCCCGTGCTGCTGCCACGACGCCGGCGTCAAGAAAGGCCCGTGGACGGAGGAGGAGGACCGCGCCCTGGTGGACCACATCCAGAGGCACGGCGGCCACGTCAGCAGCTGGCGCAACCTGCCCAAGGCCGCCGGGCTCAACCGCTGCGGCAAGAGCTGCCGCCTCCGCTGGACCAACTACCTCCGCCCCGACATCAAGCGCGGCAACTTCACCGACGACGAGGAGCGCCTCATCATCGGCCTCCATGCCCAGCTCGGTAACAGGTGGTCGACGATCGCGACGCACCTGGACGGGCGGACGGACAACGAGATCAAGAACTACTGGAACACGCACATCCGCAAGAAGCTGCTGCGCATGGGCGTCGACCCCGTCACGCACCAGCGCCTGCCCCCCGACGACCTGCTGCTCGCCGTCTCCGCCTCCCCCACGACGACGATCCTCTCGGCGGCGGCGAGCCAGCTGCGGGGCCTGAACACCGCTCTCATGCAGGTGCAAGCGCTGCAGTTTCTGCTGCAGGCCGTCAGTGGAGTTGGAAACGACGTTAACGCCGCCGCCGCTGCTGCTAATGGTGGTGGTGGTCTCGTCCCTAGCCTCAGCGCAGCCGCAGATAATAACGCCGTTCTGAACGCGAGCCGTAGCGTCGTTCCAGGCTTCCAGGACCTGCAGCTGACGACGATGACCAGGCCTCTTCTCGTGTCTCACGCGAATCACGGGTCAGCTGATCATCATCCCAGCTACATCGCAACTGATGATGTGGTGCAGCGACGACTGAACGCTTCGGCTCCTCCGGTGGTCGGCGGCGGCGCCTGTGCAGAGCCGCTGGCTGTTGCATTGGCGTCCACGGCTTATCGACACCAAGTGGCAGCTGCCGCTGACGAGCCGGTGAATGGTTTTGCCGAGCTAATACTGTCGGAGCCCATCGAGATGCCGAGCATGTGCTCTCTGGAGGACGAAGATCATTTCTGGAAGGACATGCTCGTAGAGAGCAGCAGCTTCAGCCTGCCACTA