TFome clone pUT1151

This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.

5' Primer

Name	E1 UP
Temperature (ºC)	64.7
Sequence Copy to clipboard	CAC CAT GGG GAG GGC GCC

3' Primer

Name	E1 DN
Temperature (ºC)
Sequence Copy to clipboard	GAGATTGTCGAGGAAGAAgaggt

PCR Condition

Step 1, 98C 30 sec melt, 1 cycle; Step 2, 98C 10 s

Amino Acid Sequence

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MGRAPCCGKVGLKKGRWTREEDEILARYIEEHGEGSWRSLPKNAGLLRCGKSCRLRWINYLRAGLKRGNITEEEEDVIVKLHATLGNRWSLIAGHLPGRTDNEIKNHWNSHLRRRGRAGDSRDDGVVVNIDLVKLPGGRRASSRGIVAAAKPAGKNKEKRKNGKGRNNVAEAEQQLEEEDANVSTTTPRTQSHCASAAQSEEQAQASASGLTSEEDLLALSEEMVVSALLAPGSPKLLEVGPDGSCVDGDGGLSGDSGRGSGGPSGDVAQELHLDDDAIMDWDSMGLDIPTADDTWNPLVWDYDQTSLVPEPEGEGRRQRDEMMSDLFFLDNL

TFome clones do not contain stop codon at 3'end.

CDS

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ATGGGGAGGGCGCCGTGCTGCGGGAAGGTGGGGTTGAAGAAGGGGAGGTGGACCAGGGAGGAGGACGAGATCCTGGCGAGGTACATCGAGGAGCACGGGGAAGGCTCATGGAGATCACTGCCCAAGAATGCTGGGTTGCTGCGGTGCGGGAAGAGCTGCAGGCTGCGGTGGATCAACTACCTGAGGGCGGGTCTCAAGAGGGGGAACATCACGGAGGAGGAGGAGGACGTGATCGTCAAGCTTCACGCCACGCTTGGCAACAGGTGGTCCCTGATCGCCGGTCATTTGCCTGGCCGAACTGACAACGAGATCAAGAACCACTGGAACTCGCACCTGCGCAGGCGCGGGCGGGCGGGCGACTCCCGCGACGACGGCGTCGTCGTCAACATCGACCTCGTCAAGCTGCCCGGCGGCCGCCGGGCCAGCAGCCGAGGCATCGTGGCCGCGGCCAAGCCAGCTGGAAAGAACAAGGAGAAGAGGAAGAACGGCAAGGGCAGGAACAACGTAGCAGAAGCGGAGCAGCAGCTCGAGGAGGAGGACGCCAACGTGTCAACAACTACGCCGAGGACTCAGTCTCACTGCGCCTCCGCTGCCCAGAGCGAGGAGCAAGCGCAGGCCAGCGCTAGCGGCCTGACCTCCGAGGAGGACCTGCTGGCCCTGAGCGAGGAGATGGTGGTGAGTGCGCTACTGGCCCCGGGAAGCCCGAAGCTGCTGGAGGTGGGCCCCGACGGCTCGTGCGTGGACGGTGACGGTGGCCTGTCTGGGGACAGCGGCCGTGGGTCAGGCGGGCCTTCTGGCGATGTGGCCCAGGAGCTGCACCTCGACGACGACGCCATCATGGACTGGGACTCCATGGGGCTGGACATCCCGACCGCCGATGACACGTGGAACCCCCTGGTGTGGGACTACGACCAGACGTCGTTGGTCCCGGAACCGGAAGGAGAGGGGCGCCGGCAGCGGGACGAGATGATGTCAGACCTCTTCTTCCTCGACAATCTC