TFome clone pUT1175

This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.

5' Primer

Name	C38 UP
Temperature (ºC)	63.4
Sequence Copy to clipboard	CAC CAT GGC ATC GTC GTC GCG

3' Primer

Name	C38 DN
Temperature (ºC)
Sequence Copy to clipboard	GACCTTCCACATGTTCATgttg

PCR Condition

Step 1, 98C 30 sec melt, 1 cycle; Step 2, 98C 10 s

Amino Acid Sequence

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MASSSRLDLPPGFRFHPTDEEVVSHYLTHKALDSRFSCVVIADADLNKIEPWDLPSKAKMGEKEWFFFCHKDRKYPTGMRTNRATASGYWKATGKDKEIFRGSSSSPRRVLVGMKKTLVFYTGRAPRGGKTPWVMHEYRLEGSLPHNLHRGAKDEWAVCKVINKDLAGKAGQQQMAPPPHAVSVGMERSDSLAFLDDLVLDNADDLPPLVDSTTYAAGSLFAAAGTTTTTNDDSGGYQQATKAEPQPQLPAPSNSPYQHQQQAIRRHCKAEAPAPAMVLSPSRETPGADMFQLQHVDELLQLDGGFMEDYYNMNMWKV

TFome clones do not contain stop codon at 3'end.

CDS

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ATGGCATCGTCGTCGCGGCTGGATCTCCCGCCGGGGTTCCGCTTCCACCCCACCGACGAGGAGGTGGTGTCGCACTACCTGACCCACAAGGCGCTGGACAGCCGCTTCTCCTGCGTCGTCATCGCCGACGCCGACCTCAACAAGATCGAGCCGTGGGATCTCCCAAGCAAGGCTAAGATGGGCGAGAAGGAGTGGTTCTTCTTCTGCCACAAGGACCGCAAGTACCCGACGGGGATGCGCACCAACCGCGCCACCGCCAGCGGGTACTGGAAGGCCACGGGGAAGGACAAGGAGATCTTCCGCGGCAGTAGCAGTAGTCCCCGCCGCGTGCTCGTCGGCATGAAGAAGACGCTCGTCTTCTACACGGGCCGCGCGCCGCGCGGAGGCAAGACGCCGTGGGTCATGCACGAGTACCGCCTCGAGGGCAGCCTGCCGCACAACCTCCACCGCGGGGCCAAGGACGAATGGGCTGTGTGTAAGGTGATCAACAAAGACTTGGCGGGCAAGGCTGGGCAACAACAAATGGCGCCGCCGCCGCACGCCGTGTCCGTGGGCATGGAGCGCAGCGACTCGCTGGCCTTCCTCGACGACCTGGTGCTCGACAACGCCGACGACCTGCCCCCGCTCGTCGACTCGACAACGTACGCCGCCGGCTCCCTCTTCGCCGCGGCGGGGACGACCACGACGACGAACGACGACAGCGGCGGGTACCAACAAGCCACCAAGGCGGAGCCGCAGCCGCAGCTGCCCGCGCCGAGCAACAGCCCGTACCAGCACCAGCAGCAGGCCATACGGAGGCACTGCAAGGCGGAGGCGCCGGCGCCGGCGATGGTGCTGAGCCCGTCGCGCGAGACGCCGGGCGCGGACATGTTCCAGCTCCAGCATGTGGACGAGCTGCTCCAGCTGGACGGCGGCTTCATGGAGGACTACTACAACATGAACATGTGGAAGGTC