TFome clone pUT1177

This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.

5' Primer

Name	C18 UP
Temperature (ºC)	65.2
Sequence Copy to clipboard	caccATGGGGCGCGAGGC

3' Primer

Name	C18 DN
Temperature (ºC)
Sequence Copy to clipboard	GAGGAAGTCGACGCTGAA

PCR Condition

Step 1, 98C 30 sec melt, 1 cycle; Step 2, 98C 10 s

Amino Acid Sequence

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MGREAAATRPPKLRRGLWSPEEDEKLYNHIIRYGVGCWSSVPKLAGLERCGKSCRLRWINYLRPDLKRGSFSQQEEDLIVSLHKILGNRWSQIASQLPGRTDNEIKNFWNSCIKKKLRQQGIDPATHKPLNDDDNAAAAAAADNDAAVAPHHHRRHQDDHCGAANIDNNNNNNNNNNNRQLPSADDHCFAMSSDDPLAPHSPTVSFDPLSVTNVPTAMQQGSYGSFRSDNDHLCDYGGGGVDVVSDAATAYSAPYTGGGGGGGDSSSNSNGNGTWACSGGEPMPPHVAMFGRDAAQAAAYHQFVDPAKYSPWQQHPAARLHDHNVGGAAAGFPIRSMSRDLPGSCFDLARSALEDEFSVDFL

TFome clones do not contain stop codon at 3'end.

CDS

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ATGGGGCGCGAGGCGGCAGCCACGAGGCCGCCCAAGCTGCGGAGGGGCCTGTGGTCGCCGGAGGAGGACGAGAAGCTGTACAATCACATCATCCGATATGGCGTCGGCTGCTGGAGCTCAGTCCCAAAGCTCGCTGGCTTGGAGAGATGCGGCAAGAGCTGCAGGCTGAGATGGATCAACTACCTGCGGCCTGACCTCAAGAGGGGCAGCTTCTCCCAGCAGGAGGAGGATCTCATCGTCAGCCTCCACAAGATACTAGGCAACAGGTGGTCCCAGATCGCGTCGCAGCTGCCGGGGAGGACGGACAACGAGATCAAAAACTTCTGGAACTCGTGCATCAAGAAGAAGCTCCGGCAGCAGGGCATCGACCCCGCCACCCACAAGCCGCTCAACGACGACGACAACGCTGCTGCTGCTGCTGCTGCGGACAACGACGCCGCCGTGGCGCCGCACCACCATCGTCGTCATCAGGACGACCACTGCGGCGCCGCCAATATTGATAATAATAATAATAACAACAACAACAACAACAATCGGCAGCTCCCCTCAGCCGACGACCACTGCTTCGCCATGAGCAGCGACGACCCTCTCGCGCCACACTCCCCGACCGTCAGCTTCGACCCACTGTCCGTCACCAACGTCCCCACGGCAATGCAGCAGGGCTCCTACGGCTCGTTCCGCTCCGACAACGACCACCTCTGCGACTACGGCGGCGGCGGCGTCGACGTGGTCTCGGACGCCGCCACGGCGTACAGCGCCCCCTACACAGGCGGCGGCGGCGGCGGCGGAGACAGCTCCAGCAACAGCAACGGCAACGGCACCTGGGCCTGCAGCGGCGGCGAGCCGATGCCGCCGCACGTGGCCATGTTCGGCCGCGATGCTGCCCAGGCGGCGGCGTACCATCAGTTCGTCGACCCGGCCAAGTACAGCCCCTGGCAGCAGCACCCGGCGGCGAGGCTCCATGATCACAACGTCGGCGGCGCTGCTGCGGGCTTCCCGATACGGTCCATGTCGCGGGACCTGCCGGGTTCCTGCTTCGACCTCGCCCGCAGCGCGCTGGAGGACGAGTTCAGCGTCGACTTCCTC