| TFome pUT1762 | |
|---|---|
| Template | DV540435 |
| Genbank Insert ID | HQ858776 |
| Protein Name | ZmOrphan149 |
| Species | Zea mays |
| TF Family | Orphans |
| Gene id | GRMZM2G116475 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | c.C807T p.F269(=) Not in functional domain. EST does not cover SNP. |
| Request Information | Deposited to ABRC |
| Subgenome: | 2 |
TFome clone pUT1762
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | B196UP |
| Temperature (ºC) | 69.2 |
| Sequence
|
caccATGCAGGTGCTCTGCGACGTCTGCG |
3' Primer
| Name | B196DN |
| Temperature (ºC) | |
| Sequence
|
AAAGCACCAGAAAGTTGGCG |
PCR Condition
Step 1, 98C 30 sec melt, 1 cycle; Step 2, 98C 10 s
Amino Acid Sequence
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MQVLCDVCGGAPAAVLFCTDEAALCSACDRRVHRADKRRRIPLVQPCGDDSAAAAAAPLCDVCKERRGLVFCVEDRAILCPDCDDPIHSANDLTAKHTRFLLVGAKLSAALVDQAPPSPDDDDDDVAEPDAVPAACAAKASSLESGGGSSISDYLTNICPGWRVDDLLLDDSSFSAPSKTGYSDGHDQVPSVDADLFDVVASGRPGKRVGGAALGFDKAPASVVVVPTQGCVTERTWNSDSDVFAVPELPQPPPAKKARPAPAPTFWCF
TFome clones do not contain stop codon at 3'end.
CDS
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ATGCAGGTGCTCTGCGACGTCTGCGGCGGCGCGCCGGCCGCCGTGCTCTTCTGCACTGACGAGGCCGCGCTCTGCTCCGCCTGCGACCGCCGCGTCCACCGCGCCGACAAGCGCCGCCGCATCCCGCTCGTCCAGCCCTGCGGCGACGACTCCGCCGCCGCCGCCGCCGCGCCGCTCTGCGACGTCTGCAAGGAGCGGAGGGGCCTCGTGTTCTGCGTGGAGGACCGCGCCATCCTGTGCCCCGACTGCGACGACCCCATCCACAGCGCCAACGACCTCACCGCCAAGCACACCCGCTTCCTCCTCGTCGGGGCCAAGCTCTCCGCCGCGCTCGTCGACCAGGCGCCCCCCTCCCCTGACGACGACGACGACGACGTCGCCGAGCCCGACGCTGTCCCCGCCGCCTGCGCGGCCAAGGCCTCGTCTCTTGAGTCCGGCGGCGGCAGCAGCATCTCCGACTACCTCACCAACATCTGCCCGGGCTGGCGCGTCGACGATCTTCTCTTGGACGACTCGTCGTTCTCAGCGCCATCTAAGACCGGCTACAGCGACGGCCACGATCAGGTGCCGTCCGTGGATGCCGACCTCTTCGACGTGGTGGCCAGCGGCCGGCCAGGCAAGCGCGTCGGCGGCGCAGCGCTGGGCTTCGACAAGGCCCCGGCCTCCGTTGTGGTCGTCCCGACGCAGGGGTGCGTGACGGAGAGGACTTGGAACAGCGACAGCGACGTGTTCGCGGTGCCGGAGCTCCCGCAGCCGCCGCCGGCCAAGAAGGCGCGGCCGGCGCCGGCGCCAACTTTCTGGTGCTTT
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