TFome clone pUT1766

This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.

5' Primer

Name	B172UP
Temperature (ºC)	64.4
Sequence Copy to clipboard	caccATGGCCTCGGACCAACAGG

3' Primer

Name	B172DN
Temperature (ºC)
Sequence Copy to clipboard	ATGCCCAGCCGCTGCAGCATACT

PCR Condition

Step 1, 98C 30 sec melt, 1 cycle; Step 2, 98C 10 s

Amino Acid Sequence

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MASDQQALPVPVPVPVPPNPNPTAPADLTPPSASAARKLPIKRRSPRPSSSPPSSSSPASSDPLRAPGGGGGSDQQQPPFKFQRIWSESDELRFLQGLLGCGTQGLVFPRDLNVFYDRFSESMPQPYTRAQLSEKLRRLKNKFRSMSARVAGGIDPARLAPHDRDVLHLCSRLWDPANAATSPFAASAGTSGNKRRRSNPRGTPPDASGDSNSHDYNGISSSAPGLFPDGSNGEDTFYLEQESGQLGNHEGAALVADSKFDVVVQEQPEAVVTLPNGNNGIGSEMSAECKTVVPCSNEHRMANAVLDVFEECLREAKSNGIINGGNVESELSKRWRAQRMDELDVLSRRLRLLVEYAAAAGH

TFome clones do not contain stop codon at 3'end.

CDS

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ATGGCCTCGGACCAACAGGCCCTCCCGGTCCCGGTCCCGGTCCCCGTCCCGCCCAACCCTAACCCGACCGCACCAGCCGACCTGACCCCGCCCTCGGCGTCCGCCGCGCGCAAGCTCCCCATCAAGCGGCGCTCCCCGCGGCCGTCGTCCTCGCCCCCGTCCTCTTCTTCCCCGGCCTCCTCCGACCCGCTCCGCGCGCCCGGCGGCGGCGGCGGATCCGACCAGCAGCAGCCGCCCTTCAAGTTCCAGCGCATCTGGTCCGAGTCCGACGAGCTCCGCTTCCTGCAGGGCCTCCTCGGCTGCGGCACGCAGGGCCTCGTCTTCCCGCGCGACCTCAACGTCTTCTACGACCGCTTCTCCGAGTCCATGCCCCAGCCCTACACCCGCGCCCAGCTCTCGGAGAAGCTCCGCCGCCTCAAGAACAAGTTCCGCAGCATGTCCGCGCGCGTCGCGGGAGGCATAGACCCGGCCCGCCTCGCGCCGCACGACCGCGACGTGCTCCACCTCTGCTCCAGGCTGTGGGACCCCGCCAACGCCGCCACCTCGCCCTTCGCGGCCAGCGCCGGAACGTCGGGGAACAAGCGCCGCCGCTCCAACCCGCGTGGCACGCCCCCAGACGCGTCTGGGGATAGCAACTCCCATGACTACAATGGGATTAGCTCCTCTGCTCCCGGTTTGTTTCCGGATGGTTCCAACGGCGAGGATACGTTTTACCTTGAGCAAGAAAGCGGGCAGCTGGGCAATCATGAAGGCGCTGCTCTTGTAGCAGATAGCAAATTCGATGTTGTGGTGCAGGAGCAGCCTGAGGCCGTGGTTACTCTCCCGAATGGGAACAACGGAATTGGCAGTGAGATGAGTGCGGAGTGTAAAACGGTGGTGCCGTGCAGCAACGAGCACCGAATGGCAAATGCTGTCCTAGATGTGTTTGAGGAATGCCTGAGGGAGGCAAAGAGTAACGGGATTATCAATGGTGGCAATGTGGAGAGCGAGCTCTCCAAGCGGTGGAGAGCACAGAGGATGGATGAGCTTGATGTCTTGAGCCGGAGGCTGAGGTTGCTCGTCGAGTATGCTGCAGCGGCTGGGCAT