| TFome pUT3040 | |
|---|---|
| Template | BT067260.1 |
| Genbank Insert ID | JX428446 |
| Protein Name | ZmTUB2 |
| Species | Zea mays |
| TF Family | TUB |
| Gene id | GRMZM5G866954 |
| transcript | T02 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
TFome clone pUT3040
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | UT2916up |
| Temperature (ºC) | 60.3 |
| Sequence
|
caccATGTCATTTCGTAGCATAGTCCGTGATG |
3' Primer
| Name | UT2916dn |
| Temperature (ºC) | |
| Sequence
|
TTCACAGGCTAACTTTGTATCAAAGCTACTC |
PCR Condition
Step 1, 98°C 30 sec melt, 1 cycle; Step 2, 98&
Amino Acid Sequence
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MSFRSIVRDVRDGFGSLSRRSFEVTLASIYGLTGHHKGKTQSSSHVLDDSPSIIRESRWANLPPELIRDIIRRLEADESTWPARKHVVCFAAVCRTWREMCKEIVLSPEFCGKLTFPVSLKQPGPRDGNTMVQCFIKRNKSKSTYHLYLCLSNVVTSESGKFLLSAKRHRKTTCTEYTISMDSGNISRSKRTYIGKIRSNFLGTRFLIYDTQPPYNGAVVPPAGRTSRRFNSTKVSPKLPSVSYNIAQVSYELNVLGTRGPRRMRCIMHSIPASSVEPGGTVPGQPEQIVPRALEDSFRSTASFSQSFRSTASFSKSIMDSSMDFNSARFSDIAGSSARFTGVPGSSARFAGVPGSSARFSGIAGARLDNDEESETKERPLVLRNKPPRWHEQLQCWCLNFRGRVTIASVKNFQLIAAATPPPAGAPTPSQPAPSDPDKVILQFGKVARDMFTMDYRYPLSAFQAFAICLSSFDTKLACE
TFome clones do not contain stop codon at 3'end.
CDS
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ATGTCATTTCGTAGCATAGTCCGTGATGTTAGGGATGGCTTCGGCAGCTTGTCAAGGAGAAGCTTTGAGGTGACCCTCGCAAGCATTTATGGCCTTACTGGGCATCACAAAGGGAAGACCCAGAGCTCATCACATGTGCTCGACGACTCACCTTCCATAATTCGTGAAAGTCGATGGGCGAATCTACCTCCTGAACTCATCCGTGATATCATACGGAGACTGGAGGCTGATGAGAGCACCTGGCCAGCGCGGAAGCACGTCGTTTGCTTCGCAGCTGTTTGTAGGACATGGAGAGAAATGTGTAAAGAGATTGTGTTGAGCCCGGAGTTTTGTGGCAAGCTCACCTTCCCTGTGTCTCTAAAACAGCCTGGTCCTCGAGATGGAAATACAATGGTCCAGTGTTTTATAAAGAGGAATAAGTCAAAATCCACTTACCATCTCTACCTGTGCCTTAGCAATGTTGTTACTTCAGAAAGTGGGAAATTCCTCTTATCAGCTAAACGACACCGCAAAACCACATGCACCGAGTACACTATATCAATGGATTCTGGCAACATCTCAAGATCAAAAAGAACCTACATTGGAAAAATAAGGTCTAACTTCCTTGGCACAAGATTTTTAATTTATGATACACAACCGCCCTATAATGGGGCTGTAGTTCCTCCTGCCGGAAGGACAAGCAGGAGGTTCAACTCCACAAAAGTCTCTCCAAAGCTGCCTTCAGTCAGTTACAACATCGCACAGGTATCGTATGAGTTAAATGTCCTTGGCACGAGAGGTCCTAGGCGGATGCGTTGCATCATGCACTCCATACCTGCATCATCAGTGGAGCCAGGTGGCACAGTGCCTGGACAGCCAGAGCAGATTGTACCTCGAGCCCTGGAGGATTCATTCCGCAGCACGGCTTCCTTCTCGCAGTCGTTCCGTAGCACTGCCTCATTCTCCAAGTCCATCATGGACTCATCCATGGATTTCAATAGCGCTCGCTTCTCTGACATCGCTGGCAGCAGCGCTCGCTTCACGGGCGTTCCTGGTAGCAGCGCTCGCTTCGCGGGCGTTCCTGGCAGCAGCGCTCGCTTCTCGGGCATTGCTGGCGCCAGACTGGACAACGACGAGGAGAGTGAAACCAAGGAGAGGCCACTGGTGCTTCGTAATAAGCCGCCTAGGTGGCACGAACAGCTGCAGTGTTGGTGCCTCAACTTCCGTGGCCGTGTGACAATTGCCTCGGTGAAGAACTTCCAGCTGATCGCCGCGGCTACCCCGCCACCGGCAGGTGCGCCAACCCCCTCTCAGCCTGCCCCGTCAGATCCCGACAAGGTGATTCTCCAGTTTGGCAAAGTGGCAAGGGATATGTTCACGATGGACTATCGCTACCCTCTCTCGGCTTTCCAGGCCTTTGCCATTTGTCTGAGTAGCTTTGATACAAAGTTAGCCTGTGAA
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