TFome clone pUT3085

This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.

5' Primer

Name	UT2503up
Temperature (ºC)	61.5
Sequence Copy to clipboard	caccATGAACTCCATGGAATCATGCGTGCC

3' Primer

Name	UT2503dn
Temperature (ºC)
Sequence Copy to clipboard	TTTGTCGAACACACACAGTCCAAAGTCG

PCR Condition

Step 1, 98°C 30 sec melt, 1 cycle; Step 2, 98&

Amino Acid Sequence

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MNSMESCVPPGFRFHPTDEELVGYYLRKKVAAQKIDLHVIRDIDLYRIEPWDLQGHCGIGYEEQNEWYFFSYKDRKYPTGTRTNRATMAGFWKATGRDKAVHDKSRIIGMRKTLVFYKGRAPNGHKTDWIMHEYRLETDENAPPQEEGWVVCRAFKKRTAYPARMAWGDPSYAYREISAMGAAAAAFVDPNAASYAQIRRQQSNKSARFKQEAELDGGAAAALLQYSSSHLVELPQLESPSAPANKSQAASASDEVVDAADSGRRPGKKARADEVATDWRALDKFVASQLSPAAECGLEAAAASSTAAASNVGSQLDHVEDDDMAALLFLNSDGRDEAERWTGLLGPAAGDDGDFGLCVFDK

TFome clones do not contain stop codon at 3'end.

CDS

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ATGAACTCCATGGAATCATGCGTGCCCCCGGGTTTCAGGTTCCACCCCACCGACGAGGAGCTCGTCGGCTACTACCTCAGGAAGAAGGTGGCCGCCCAGAAGATCGACCTCCACGTCATACGCGACATCGACCTCTACCGCATCGAGCCATGGGATCTCCAAGGGCATTGCGGGATCGGGTACGAGGAGCAAAACGAGTGGTACTTCTTCAGCTACAAGGACCGCAAGTACCCCACGGGGACGCGGACCAACCGGGCCACCATGGCCGGCTTCTGGAAGGCCACGGGCAGGGACAAGGCGGTGCACGACAAGAGCCGCATCATCGGCATGAGGAAGACGCTCGTCTTCTACAAGGGGAGGGCCCCCAACGGCCACAAGACCGACTGGATCATGCACGAGTACCGCCTCGAGACCGACGAGAATGCGCCGCCGCAGGAAGAAGGCTGGGTGGTGTGCCGAGCGTTCAAGAAGAGAACAGCGTACCCGGCAAGGATGGCATGGGGGGACCCCAGCTACGCCTACCGCGAGATCAGCGCCATGGGCGCGGCGGCGGCGGCGTTCGTGGACCCTAACGCGGCGTCGTACGCGCAGATCAGACGCCAGCAGTCCAACAAGAGCGCGCGCTTCAAGCAGGAGGCCGAGCTGGACGGCGGCGCCGCCGCCGCGCTGCTGCAGTACTCCTCCAGCCACCTGGTCGAGCTGCCGCAGCTCGAGAGCCCGTCGGCGCCGGCCAACAAGAGCCAGGCGGCGTCAGCCTCTGATGAGGTTGTGGACGCCGCCGACAGCGGCCGGCGGCCGGGCAAGAAGGCGCGGGCTGATGAAGTGGCCACGGACTGGAGGGCGCTCGACAAGTTCGTCGCGTCGCAGCTCAGCCCCGCGGCGGAGTGCGGCTTGGAGGCGGCGGCAGCGTCGTCGACGGCCGCCGCCAGCAACGTGGGCTCCCAGCTGGACCACGTTGAGGATGATGACATGGCGGCATTGCTGTTCCTCAACAGCGACGGGAGAGACGAGGCGGAGAGGTGGACGGGGCTGCTCGGCCCGGCCGCCGGGGACGACGGCGACTTTGGACTGTGTGTGTTCGACAAA