| TFome pUT3150 | |
|---|---|
| Template | BT054809.1 |
| Genbank Insert ID | JX469907 |
| Protein Name | ZmMYB133 |
| Species | Zea mays |
| TF Family | MYB |
| Gene id | GRMZM2G149958 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
| Subgenome: | 1 |
TFome clone pUT3150
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | B287_F |
| Temperature (ºC) | 65.5 |
| Sequence
|
caccATGGCCGTGGACGAGGC |
3' Primer
| Name | B287_R |
| Temperature (ºC) | |
| Sequence
|
CCCATGAGATGGTGGGGG |
PCR Condition
40 sec 98°C, 30x(15sec 98°C, 30sec 45°
Amino Acid Sequence
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MAVDEASSSGGGEEGCGSWTREQEKAFENAVATMGGEEDGDARWEKLAEAVEGKTPEEVRRHYELLVEDVDGIESGRVPLPAYAADGAAEEGGGGGKKGSGGGGTHGDKGSAKSAEQERRKGIAWTEDEHRLFLLGLEKYGKGDWRSISRNFVISRTPTQVASHAQKYFIRLNSMNRERRRSSIHDITSVNNGDPSTAQGPITGQTNGQAANPGKPSKQSPQPANTPPGVDAYGTTIGQPVGGPLVSAVGTPVTLPVSAPPHLAYGMRAPVPGAVVPGAPVNIAPMPYPMPPPPSHG
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGCCGTGGACGAGGCGAGCAGCAGTGGCGGTGGGGAGGAGGGGTGCGGGTCGTGGACCCGCGAGCAGGAGAAGGCGTTCGAGAACGCGGTGGCGACGATGGGCGGGGAGGAGGACGGGGACGCGAGGTGGGAGAAGCTAGCGGAGGCCGTCGAGGGGAAGACGCCGGAGGAGGTCAGGCGGCACTACGAGCTGCTGGTGGAGGACGTCGACGGCATCGAGTCGGGCCGCGTCCCGCTTCCGGCGTACGCGGCTGACGGCGCTGCCGAGGAAGGGGGCGGTGGCGGGAAGAAGGGAAGTGGCGGAGGAGGGACCCATGGGGACAAGGGGTCGGCGAAGTCCGCCGAGCAGGAGCGTAGGAAGGGCATCGCCTGGACAGAGGACGAGCACAGATTGTTCCTCCTTGGACTTGAAAAGTATGGCAAAGGTGACTGGCGGAGCATCTCGCGGAACTTTGTGATCTCAAGGACGCCGACTCAAGTAGCTAGTCATGCACAGAAGTACTTTATCCGTCTGAACTCAATGAACAGAGAAAGGCGGCGATCAAGTATACATGACATCACCAGTGTGAACAATGGAGATCCATCAACTGCACAGGGTCCAATCACAGGCCAAACAAATGGTCAGGCTGCAAATCCTGGAAAACCATCTAAGCAATCCCCGCAGCCAGCGAATACACCTCCAGGCGTCGATGCTTATGGCACAACAATTGGACAGCCAGTTGGTGGCCCCCTTGTCTCAGCTGTTGGAACTCCCGTTACACTACCTGTTTCCGCTCCACCTCATCTGGCCTATGGCATGCGTGCCCCGGTTCCAGGAGCCGTAGTACCTGGTGCTCCAGTAAACATTGCTCCGATGCCCTATCCAATGCCACCCCCACCATCTCATGGG
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