| TFome pUT3166 | |
|---|---|
| Template | BT035304.1 |
| Genbank Insert ID | KJ726810 |
| Protein Name | ZmWRKY53 |
| Species | Zea mays |
| TF Family | WRKY |
| Gene id | GRMZM2G125653 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
| Subgenome: | 1 |
TFome clone pUT3166
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | B352_F |
| Temperature (ºC) | 67.9 |
| Sequence
|
caccATGATGCTGCTCATGGACTCG |
3' Primer
| Name | B352_R |
| Temperature (ºC) | |
| Sequence
|
GTACTCCGGCAGCTGCTGCA |
PCR Condition
5 l GC buffer (NEB) 1 l primer forward 20mM 1 l pr
Amino Acid Sequence
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MMLLMDSGSRGDCSPVCLDLSVGLSPPSPGSGPETTADAERLDRPATGCWGPRTSSLADGKDEAKTLEARLTQVSEENRRLTEIIAYMYASQVAARRSPDGRKRSRDSLEPSNSGDANAAVESAALSDEGTCRRIKLTRVCTKIDPSDTTLTVKDGYQWRKYGQKVTRDNPSPRAYFRCAYAPSCPVKKKVQRSAEDSAMLVATYEGEHNHPSPTRAGELPSSTSINSSGPAITLDLTRNGAGAVRGLDAAAEVPGLKRLCQEIASPDFRTALVEQMARSLTKDPKFTDALAAAILQQLPEY
TFome clones do not contain stop codon at 3'end.
CDS
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ATGATGCTGCTCATGGACTCGGGGAGCCGCGGTGACTGCTCCCCGGTCTGCTTGGACCTCAGCGTCGGCCTTTCGCCGCCGTCGCCGGGGAGCGGCCCGGAAACGACAGCTGACGCTGAGAGACTCGACCGTCCCGCCACTGGCTGCTGGGGGCCACGGACATCGTCCCTCGCTGACGGGAAGGACGAGGCCAAGACCCTGGAGGCCAGGCTCACCCAGGTCAGCGAGGAGAACCGGCGTCTCACCGAGATCATCGCCTACATGTACGCCAGCCAGGTCGCCGCGCGGCGGAGTCCCGACGGCAGGAAGAGGAGCAGGGACAGCCTGGAGCCGTCGAATTCGGGCGACGCCAACGCTGCCGTCGAGAGCGCCGCCCTCAGCGACGAGGGCACGTGCAGGCGGATCAAGCTCACCAGGGTCTGCACCAAGATCGACCCCTCCGACACCACGCTCACCGTCAAAGACGGCTACCAGTGGCGCAAGTACGGCCAGAAGGTGACGCGCGACAACCCGTCCCCGAGAGCCTACTTCCGCTGCGCATACGCACCATCCTGCCCCGTCAAGAAGAAGGTGCAGAGGAGCGCGGAGGACAGCGCCATGCTGGTGGCCACGTACGAGGGCGAGCACAACCACCCGAGCCCGACGCGCGCCGGCGAGCTCCCCAGCTCCACCTCCATCAACTCCTCCGGCCCGGCCATCACGCTGGACCTCACCAGGAACGGAGCCGGCGCCGTGCGGGGGCTCGATGCCGCCGCCGAGGTGCCCGGCCTCAAGAGGCTGTGCCAGGAGATCGCGTCACCGGATTTCCGCACGGCGCTCGTGGAGCAGATGGCGCGCTCGCTCACCAAGGATCCCAAGTTCACCGACGCGCTGGCTGCCGCGATCCTGCAGCAGCTGCCGGAGTAC
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