TFome clone pUT3405

This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.

5' Primer

Name	UT3274up
Temperature (ºC)	63.2
Sequence Copy to clipboard	caccATGGCAAGCTTATTCCCACAGCTC

3' Primer

Name	UT3274dn
Temperature (ºC)
Sequence Copy to clipboard	CTGGAAAGAGCACATGTGGTTACTGAA

PCR Condition

Step 1, 98°C 30 sec melt, 1 cycle; Step 2, 98&

Amino Acid Sequence

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MASLFPQLAQMEHGVAGASHGAPSFLFCNGAGAATADSASLETSSGVLDTSPRGGGADSGGRKAPTKPREDSASASASFSFSSAHSKDSSSKGSTKNRGGKRGRSSKEVDEEEEPKGYIHVRARRGQATDSHSLAERVRRERISERMRVLQALVPGCDKVTGKALVLDEIINYVQSLQNQVEFLSMKIASLSPVLYGFGMDTTDAFSTDHTTQKIEGMLQLHHEALAVPSSALSRPPFEAIADSTHATASASSPRSYELHGDGGGGGASISFPQDGGGYMAQTVGGPRPELFNQVVFSNHMCSFQ

TFome clones do not contain stop codon at 3'end.

CDS

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ATGGCAAGCTTATTCCCACAGCTCGCGCAGATGGAGCACGGCGTAGCCGGCGCCTCCCATGGCGCGCCGAGCTTCCTGTTCTGCAATGGCGCAGGTGCAGCCACGGCAGACTCAGCCTCGCTAGAGACCTCGTCCGGTGTTCTCGACACCTCCCCACGAGGCGGAGGCGCTGATTCTGGCGGTAGGAAGGCCCCGACGAAGCCCAGGGAAGACAGCGCCAGCGCCAGCGCCAGCTTCAGCTTCAGCTCTGCGCACTCCAAGGACTCGAGCTCGAAGGGAAGCACCAAGAATAGGGGAGGGAAAAGAGGCAGAAGCAGCAAGGAGGTAGACGAGGAGGAGGAGCCCAAGGGGTACATACACGTCAGGGCAAGGAGAGGGCAAGCCACGGACAGCCACAGCCTTGCGGAGAGGGTGAGGAGGGAGAGGATCAGCGAGAGGATGAGGGTGCTGCAGGCCCTGGTCCCTGGCTGTGATAAGGTCACTGGAAAAGCCCTCGTTTTGGACGAGATCATCAACTACGTGCAGTCCTTGCAGAACCAAGTCGAGTTCCTTTCCATGAAGATCGCTTCACTGAGCCCTGTGCTGTATGGCTTTGGCATGGACACTACTGACGCTTTCAGCACTGACCATACTACTCAAAAAATCGAAGGGATGCTCCAGCTCCACCACGAGGCGCTTGCGGTGCCTAGCTCTGCCCTGAGCAGACCTCCATTCGAGGCTATCGCGGACAGTACGCACGCCACCGCCTCTGCCTCCTCCCCCCGGTCCTACGAGCTCCACGGCGATGGCGGCGGCGGCGGCGCCAGCATCTCTTTCCCGCAGGACGGCGGCGGCTACATGGCGCAAACAGTAGGTGGGCCAAGGCCAGAGCTGTTCAACCAAGTGGTGTTCAGTAACCACATGTGCTCTTTCCAG