| TFome pUT3482 | |
|---|---|
| Template | BT041237.1 |
| Genbank Insert ID | KJ726936 |
| Protein Name | ZmMYBR111 |
| Species | Zea mays |
| TF Family | MYB-related |
| Gene id | GRMZM2G181030 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | c.A530T p.Q177L Not present in template FlcDNA. Not in functional domain. |
| Request Information | Deposited to ABRC |
| Subgenome: | 2 |
TFome clone pUT3482
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | C166_F |
| Temperature (ºC) | 64.3 |
| Sequence
|
caccATGGCTCGTATTCAGCC |
3' Primer
| Name | C166_R |
| Temperature (ºC) | |
| Sequence
|
CAGGCACAGCCTTGTCATC |
PCR Condition
PCR Reaction Mix: 5 l GC buffer (NEB) 1 l primer f
Amino Acid Sequence
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MARIQPRTDQRPAVDVDHVGHGNFTGHLKSTLSSGDMDLPGGARAPKARKPYTISKQREKWTEDEHRLFLEALRQHGRAWRRIQEHVGSKTAVQIRSHAQKFFSKVIRESSGGDSNGNGNGVAAPPQIQIPPPRPKRRPTHPYPRKLGSSVGKDDASAIKRLQEPQLQAQSPSLSELETCSPKSVLTTAEGSGSPASSVYMMEDRCLTPSTAVALSKEATTSDEAACELPGGPVLWLFGKRVVVSNLFDQQPSSNTGSLQLQRVADMELDASAPESPTSCGTGKKLSSHAAEEAKTWHPWLTGTRQFMHCLPQGEQFSVHSACRPLSYGDGSMPYTAWNAQTVTSNKQQDNQPSQAAADCEVTRAQGSWTEPSVTTSSSSVVETAQNSDSVESTKVNNDGDKVAPVPGSRKCASVVPDCLRGFAPCKRTPQSKMLLQSEAPGEADREMTRLCL
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGCTCGTATTCAGCCGAGGACCGATCAAAGGCCTGCTGTGGACGTGGACCACGTCGGCCATGGAAACTTCACGGGTCATCTGAAGAGCACTCTCAGCTCCGGCGATATGGACTTGCCAGGAGGAGCACGAGCCCCTAAGGCACGCAAGCCATACACGATATCCAAGCAGAGGGAGAAATGGACCGAGGACGAGCACAGGCTCTTCTTGGAAGCCCTGCGGCAGCATGGCCGTGCCTGGCGCCGTATACAAGAGCACGTAGGCAGCAAGACTGCCGTGCAGATCAGGAGCCACGCTCAGAAGTTCTTCTCCAAGGTCATCCGAGAATCCTCTGGAGGGGATAGCAACGGCAACGGCAACGGCGTAGCGGCTCCACCACAAATTCAGATTCCGCCGCCGCGGCCCAAGAGGAGGCCAACGCACCCGTACCCGCGCAAGCTGGGTAGTTCGGTCGGCAAGGACGACGCCTCCGCGATCAAGCGGCTTCAGGAGCCCCAGCTGCAGGCACAGTCCCCGTCTCTGTCTGAGCTGGAGACTTGCTCACCGAAGTCTGTGTTAACCACAGCTGAAGGTAGTGGATCACCAGCTTCCTCGGTTTACATGATGGAGGACAGATGCCTCACACCAAGCACGGCAGTTGCACTTTCCAAGGAGGCCACCACTTCTGACGAGGCGGCGTGTGAACTACCTGGAGGTCCAGTTCTTTGGCTATTTGGCAAGAGGGTTGTGGTTAGTAATTTATTCGATCAGCAACCAAGCTCCAATACTGGGAGCCTGCAACTACAACGTGTGGCAGACATGGAACTGGACGCTTCAGCTCCTGAGTCACCGACTAGTTGTGGAACTGGGAAGAAGCTGTCCTCCCATGCCGCAGAGGAAGCGAAGACATGGCACCCGTGGCTGACCGGTACGCGGCAGTTTATGCACTGTCTTCCTCAAGGCGAGCAGTTCTCCGTGCATTCTGCTTGCCGACCCCTGAGCTACGGTGATGGAAGCATGCCTTACACGGCGTGGAACGCGCAGACAGTTACCTCAAACAAGCAGCAGGACAACCAACCGTCTCAAGCCGCAGCAGACTGCGAGGTCACGAGGGCACAAGGATCGTGGACGGAGCCGTCCGTCACAACCTCCTCCAGCAGCGTGGTTGAAACAGCTCAGAATTCAGATTCTGTAGAATCCACGAAAGTAAACAACGACGGAGACAAAGTGGCACCTGTTCCAGGTTCAAGGAAATGCGCGAGCGTAGTTCCAGACTGCCTTCGAGGTTTTGCACCGTGCAAGCGCACGCCTCAGAGCAAGATGCTGCTGCAGTCAGAGGCACCTGGGGAGGCAGATAGAGAGATGACAAGGCTGTGCCTG
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