| TFome pUT5352 | |
|---|---|
| Template | BT033269.1 |
| Genbank Insert ID | KJ727493 |
| Protein Name | ZmbZIP123 |
| Species | Zea mays |
| TF Family | bZIP |
| Gene id | GRMZM2G002075 |
| transcript | T01 |
| Vector | pENTR/D-TOPO |
| Note | c.G571A p.E191K not present in template FlcDNA. (not in functional domain) |
| Request Information | Deposited to ABRC |
| Subgenome: | 2 |
TFome clone pUT5352
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | B446_F |
| Temperature (ºC) | 75.0 |
| Sequence
|
caccATGGACGAGCTGCTCCG |
3' Primer
| Name | B446_R |
| Temperature (ºC) | |
| Sequence
|
CCAGGGGGCCGTCAACG |
PCR Condition
40 sec 98°C 30x : 15 sec 98°C 30 sec 58&de
Amino Acid Sequence
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MDELLRSIWTAEETQAKASASIAGASAGMPPTPMQRQGSSLTLPRMLSTKTVDEAWRNLVRDEPPQGADGGGHQPPHRQSTLGEMTLEEFLVRAGAVKENPASAPPAPPPMVPPRPVPVAPKSSAFFGDLPGADDDAATAAVGFAPVGMVDLALIPPRAAGMGGSAMAVQTAVSQLDSVGKGYSDLSSLTKPQTFSFERMIRGRRHGGGVEKVVERRRRRMIKNRESAARSRARKQAYTMELEAEVQKLREQNQELERKQAEIMEMQNNEVSEMLKDPFGRKKRLCLRRTLTAPW
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGACGAGCTGCTCCGGAGCATCTGGACCGCAGAGGAGACCCAGGCCAAGGCCTCCGCCTCTATCGCAGGAGCGAGCGCGGGGATGCCCCCCACGCCGATGCAGCGGCAGGGATCGTCGCTCACACTGCCCCGCATGCTCAGCACCAAGACGGTCGACGAGGCGTGGAGAAATCTCGTGCGCGACGAACCGCCGCAGGGGGCGGACGGCGGCGGACACCAGCCGCCCCACCGCCAGTCAACGCTCGGGGAGATGACTCTGGAGGAGTTCTTGGTCAGAGCTGGCGCGGTCAAGGAGAACCCCGCTTCTGCTCCTCCAGCGCCGCCACCCATGGTGCCGCCGCGGCCGGTACCTGTTGCCCCTAAAAGCTCCGCCTTTTTTGGGGATTTACCGGGTGCCGACGACGACGCAGCTACGGCAGCGGTAGGGTTTGCACCGGTCGGCATGGTGGATCTGGCCCTGATACCGCCCAGGGCGGCAGGCATGGGAGGCAGCGCCATGGCTGTGCAAACAGCGGTGAGCCAGCTTGATTCTGTCGGGAAGGGATACAGCGACCTGTCGTCGCTGACGAAGCCGCAAACTTTTTCGTTTGAAAGAATGATTCGGGGGAGAAGGCATGGAGGCGGAGTGGAGAAAGTGGTGGAGAGGCGGCGGCGGAGGATGATCAAGAACAGGGAGTCCGCCGCGAGGTCCCGAGCGCGCAAACAGGCTTATACAATGGAGTTAGAAGCCGAAGTTCAGAAACTCAGAGAGCAGAATCAGGAACTGGAGAGGAAACAGGCAGAAATTATGGAAATGCAGAACAACGAGGTATCAGAAATGTTGAAGGATCCATTCGGACGGAAGAAACGGCTGTGCTTGCGAAGAACGTTGACGGCCCCCTGG
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