| TFome pUT5359 | |
|---|---|
| Template | BT061660.1 |
| Genbank Insert ID | KJ727500 |
| Protein Name | ZmWRKY71 |
| Species | Zea mays |
| TF Family | WRKY |
| Gene id | GRMZM2G052671 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
| Subgenome: | 2 |
TFome clone pUT5359
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | B358_F |
| Temperature (ºC) | 61.6 |
| Sequence
|
caccATGTCCGGCGCAAGTAACTATG |
3' Primer
| Name | B358_R |
| Temperature (ºC) | |
| Sequence
|
CATCTGATGACCGAAAGGAGGCCT |
PCR Condition
PCR Reaction Mix: 5 l GC buffer (NEB) 1 l primer f
Amino Acid Sequence
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MSGASNYGSLIDEWLLPPTPSPRTLMSSFLNEEFSSAPFSGPHDQSEKSREVLNSSDEVPSHAVKGPFQNGFPNLFSAKSNSNGGLAERRAARAGFSVPKIDTSRVGSSTVIRSPVSIPPGLSPTTLLESPVFLYNKMAQPSPTTGTLPFLMATNDKSTISPAAKITEDSTFDNDVFSFQPHLGSEQTGFSTAEKDYGAYERKQSLSNIHQQESSLQSSFTAVKDNTSATIVKAKPSSSSMFSDSHYSADQQQADETNIKVQGKGVEARSAAFAPVSAHSDASLLESQEALDVSSTLSNEEDERATHGTVSIECDGDEADAVSKRRKLELDALEATATATTSTTNTIDMGPAASRAVREPRVVVQTTSEVDILDDGYRWRKYGQKVVKGNPNPRSYYKCTNPGCSVRKHVERASHDLKSVITTYEGKHNHEVPAARNSGQGSSSAPSASQAVGSHRRQEPAQASFAHFGTASFSSFGLPPSGQLGPTTGNFGFGMVPPGMTIPVPSLGSLAPVKMVGNSSTMQGYPGLMVPGEPKAEPVSQPLIQMANASPPAYQQILSRPPFGHQM
TFome clones do not contain stop codon at 3'end.
CDS
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ATGTCCGGCGCAAGTAACTATGGATCCCTCATCGATGAATGGTTGCTGCCCCCTACACCAAGCCCAAGAACTCTCATGTCTAGCTTCCTGAATGAAGAATTCAGCTCTGCGCCATTCTCTGGTCCCCATGATCAATCTGAAAAGAGCAGAGAAGTTTTGAATTCGAGCGATGAGGTTCCTTCACATGCTGTCAAAGGCCCATTTCAAAATGGTTTCCCAAATTTGTTCAGTGCTAAATCAAACTCCAATGGTGGTTTGGCGGAGCGCAGGGCTGCGAGAGCAGGTTTCAGTGTCCCGAAAATTGATACTTCTCGAGTTGGTTCATCCACAGTTATTCGATCTCCTGTGTCAATTCCACCTGGTCTAAGTCCAACTACACTTCTTGAGTCTCCTGTTTTTCTTTACAATAAAATGGCACAACCGTCTCCAACCACTGGCACATTGCCATTTTTGATGGCTACGAATGATAAGTCAACAATATCACCAGCTGCCAAGATAACTGAAGATTCTACATTTGATAATGATGTGTTTTCTTTCCAACCCCACTTAGGTTCTGAACAAACAGGGTTCTCTACTGCAGAAAAGGACTATGGTGCCTATGAGAGAAAGCAGTCCTTGTCGAATATTCATCAGCAGGAATCCAGTCTCCAGTCAAGCTTTACCGCAGTCAAGGATAACACTAGTGCAACAATTGTTAAAGCGAAGCCATCTTCTAGTTCCATGTTCAGTGATAGTCACTATTCAGCTGATCAACAGCAAGCTGACGAGACAAACATAAAGGTGCAAGGCAAAGGTGTGGAGGCTAGATCAGCTGCTTTTGCTCCTGTATCAGCGCACAGTGATGCATCTCTCCTGGAGTCTCAAGAAGCACTTGATGTCTCCTCAACACTGTCTAATGAAGAGGACGAGAGGGCAACGCATGGCACTGTTTCTATAGAGTGTGATGGTGATGAAGCTGATGCTGTATCTAAAAGAAGGAAGCTGGAACTAGATGCTTTAGAAGCTACTGCTACTGCTACTACCTCCACCACCAATACCATCGACATGGGGCCTGCAGCCTCAAGAGCTGTCCGGGAGCCTAGGGTTGTTGTTCAGACCACAAGTGAGGTTGACATTCTTGACGACGGTTATCGGTGGCGCAAGTATGGGCAGAAGGTTGTTAAGGGCAATCCAAATCCAAGGAGCTACTACAAATGTACAAACCCGGGCTGTTCAGTGCGCAAGCATGTGGAAAGAGCATCACACGATCTGAAATCAGTCATCACAACGTATGAGGGAAAGCACAACCATGAAGTTCCAGCAGCCAGAAATAGTGGGCAAGGCAGTTCCAGCGCTCCATCTGCATCACAAGCTGTTGGTTCTCATCGTAGGCAAGAACCTGCACAGGCCAGCTTTGCTCACTTTGGTACGGCCTCTTTCAGCTCGTTCGGTCTCCCACCGAGTGGTCAGTTGGGACCAACAACTGGCAATTTCGGCTTCGGGATGGTTCCGCCAGGCATGACGATCCCAGTGCCCTCTCTAGGATCACTTGCCCCTGTAAAGATGGTTGGAAATTCATCAACTATGCAGGGCTACCCAGGGCTTATGGTGCCGGGAGAACCAAAGGCAGAGCCTGTCTCGCAACCACTTATCCAAATGGCAAATGCATCTCCACCAGCTTACCAACAGATATTGAGCAGGCCTCCTTTCGGTCATCAGATG
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