TFome clone pUT5365

This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.

5' Primer

Name	C150_F
Temperature (ºC)	65.5
Sequence Copy to clipboard	caccATGGAGGGCGGCGATGG

3' Primer

Name	C150_R
Temperature (ºC)
Sequence Copy to clipboard	GTCCTGGCCGTTCGCGGCACTACC

PCR Condition

PCR Reaction Mix: 5 l GC buffer (NEB) 1 l primer f

Amino Acid Sequence

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MEGGDGCGSSAAASGSGRVTRRWTPEEDDLLRRAVARHGPRKWPELSVDIPGRSGRSCHRRWVQHLSPGLERRAFTPEEDAVIVAAQAMYGNKWTTIARMLPGRTRTDISIKNRWNSALQQHCRAATASANTAGCALPLRPATTAPVPIPDLMYLNKKLAAAEASLPFPRLEPKDDEDDEDMDETGEDEDMNELGDDGSSEVFPPVPAVPLTLTLSLSSGVHGTLASQSTFASAATLASAATLASQSTLASQSTFASAAATAGSAMTMRSNLGQDPWLVPALRLIVSDEVQRKMQEMPQVTAPASRSSADAADGSAANGQD

TFome clones do not contain stop codon at 3'end.

CDS

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ATGGAGGGCGGCGATGGCTGCGGCTCCTCGGCGGCGGCGTCGGGGTCCGGGAGGGTGACGCGGCGGTGGACGCCGGAGGAGGACGACCTGCTGCGGCGCGCGGTCGCGCGGCACGGGCCGCGGAAGTGGCCCGAGCTCAGCGTCGATATCCCCGGTCGGTCCGGAAGGTCGTGCCACCGGCGGTGGGTCCAGCATCTCAGCCCCGGCCTGGAGCGTCGCGCCTTCACGCCCGAGGAGGACGCCGTCATCGTCGCGGCGCAAGCGATGTACGGCAACAAGTGGACCACCATCGCGCGGATGCTCCCCGGCCGCACCCGCACCGATATCTCCATCAAGAACCGCTGGAACTCCGCGCTGCAACAACATTGCAGGGCGGCGACCGCCAGCGCCAACACCGCCGGGTGCGCCCTCCCACTGCGGCCGGCGACCACGGCCCCCGTGCCGATTCCCGATCTCATGTATCTCAACAAGAAGTTGGCGGCGGCTGAGGCATCCCTTCCGTTCCCTCGTCTGGAGCCAAAGGACGACGAGGACGACGAGGACATGGACGAGACGGGCGAGGACGAGGACATGAACGAGCTGGGGGACGACGGCAGCAGCGAGGTCTTCCCGCCCGTGCCGGCGGTGCCGCTCACGCTAACCCTCAGCCTCTCTTCCGGCGTCCACGGCACCCTCGCATCGCAGAGCACCTTCGCATCTGCGGCGACCCTCGCATCGGCGGCTACCCTCGCATCGCAGAGCACCCTCGCATCGCAGAGCACCTTCGCATCGGCGGCGGCGACAGCGGGGAGTGCCATGACAATGAGGTCGAATCTTGGGCAGGATCCGTGGTTGGTGCCTGCGTTGCGGCTGATTGTTAGCGATGAGGTGCAGCGGAAGATGCAGGAGATGCCGCAGGTCACGGCTCCGGCCAGCAGGAGCAGCGCCGATGCTGCCGACGGTAGTGCCGCGAACGGCCAGGAC