| TFome pUT5435 | |
|---|---|
| Template | BT067017 |
| Genbank Insert ID | KJ727575 |
| Protein Name | ZmHB115 |
| Species | Zea mays |
| TF Family | Homeobox |
| Gene id | GRMZM2G021339 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | c.A605G p.E202G SNP present in template FlcDNA, not in functional domain. |
| Request Information | Deposited to ABRC |
| Subgenome: | 2 |
TFome clone pUT5435
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | B773_F |
| Temperature (ºC) | 64.1 |
| Sequence
|
caccATGGAGTCCGGACGGCTC |
3' Primer
| Name | B773_R |
| Temperature (ºC) | |
| Sequence
|
GTTCCACATCCACCACCAGCTG |
PCR Condition
40 sec 98°C 30x : 15 sec 98°C 30 sec 60&de
Amino Acid Sequence
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MESGRLIFNAPGSGAGQMLFLDCGAGGGPGGGLLHRGGRPMLGLEEGRGVKRPFYTSPDELLEEEYYDEQLPEKKRRLTPEQVHLLERSFEEENKLEPERKTELARKLGLQPRQVAVWFQNRRARWKTKQLERDFDRLKASFDALRADHDALLQDNHRLRSQVVSLTEKLREKEATEGDANGAAAALPAVGVVRASVADDVGEPEPEPEPEAEAAAFEERQVKSEDRLSTGSGGSAVVDAADALLYNGRIGGAAVDSSVESYFPGAEVQDQYHGCGTMGPVTHGAGGIQSDDDDGAGSDEGCSYYADEEAAAAFLSEHTHHADGDDEDAGQVSWWWMWN
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGAGTCCGGACGGCTCATCTTCAACGCGCCCGGCTCCGGCGCCGGGCAGATGCTCTTCCTGGACTGCGGCGCAGGCGGTGGTCCCGGCGGCGGCTTGTTGCATCGAGGCGGGAGACCGATGCTTGGCCTTGAAGAAGGGCGCGGCGTAAAACGGCCCTTCTACACCTCGCCCGACGAGCTCCTCGAGGAGGAGTACTACGACGAGCAGCTGCCGGAGAAGAAGCGCCGCCTGACGCCGGAGCAGGTGCACCTGCTGGAGAGGAGCTTCGAGGAGGAGAACAAGCTGGAGCCGGAGCGCAAGACGGAGCTGGCGCGCAAGCTGGGCCTGCAGCCCCGGCAGGTGGCCGTGTGGTTCCAGAACCGGCGCGCCCGGTGGAAGACCAAGCAGCTGGAGCGCGACTTCGACCGCCTCAAGGCCTCCTTCGACGCCCTCCGCGCGGACCACGACGCCCTTCTCCAGGACAACCACCGCCTCCGCTCACAGGTGGTGTCGTTGACCGAGAAGCTGCGAGAGAAGGAGGCGACGGAGGGCGACGCCAACGGCGCCGCGGCGGCGTTGCCGGCGGTGGGCGTTGTCAGGGCTTCCGTGGCCGATGACGTCGGGGAACCGGAGCCGGAGCCGGAGCCGGAGGCAGAGGCGGCGGCGTTCGAGGAGCGGCAGGTGAAGTCCGAGGACAGGCTGAGCACGGGCAGCGGCGGGAGCGCGGTGGTGGACGCGGCGGACGCGCTGTTGTACAACGGCAGGATCGGCGGCGCGGCCGTCGATAGCAGCGTCGAGTCGTACTTCCCCGGCGCCGAGGTCCAGGACCAGTACCACGGCTGCGGGACGATGGGTCCAGTAACCCACGGCGCTGGAGGCATCCAGTCGGACGACGACGACGGCGCCGGCAGCGACGAGGGATGCAGCTACTACGCCGACGAAGAAGCCGCCGCCGCGTTCCTCTCCGAACACACCCACCACGCGGACGGCGACGACGAGGACGCCGGCCAGGTCAGCTGGTGGTGGATGTGGAAC
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