| TFome pUT5467 | |
|---|---|
| Template | BT034468.1 |
| Genbank Insert ID | KJ727607 |
| Protein Name | ZmHB84 |
| Species | Zea mays |
| TF Family | Homeobox |
| Gene id | GRMZM2G099319 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
| Subgenome: | 1 |
TFome clone pUT5467
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | C303_F |
| Temperature (ºC) | 62.8 |
| Sequence
|
caccATGGCTACCTACTACTCGAGCCA |
3' Primer
| Name | C303_R |
| Temperature (ºC) | |
| Sequence
|
CGCCACAAAATCATGGAGAAGGG |
PCR Condition
PCR Reaction Mix: 5 l GC buffer (Thermo Fisher/ Fi
Amino Acid Sequence
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MATYYSSQGSEREPSQNMYSRDPGNGTASYPPLPSALGNLLYLNSPASGGPYTDFGGVQQDCIEMPEPGHPSVMSHDSSAREIEPDMLGQQRRSFGLARGMKDEVLMRMMDGSGSHSSAADAIHDDAPNGIQLDFGVLGSHGPPAQGGQGSLSLSLNTHIMAPALPYWPVVKADMLSPHSYDHGCGGGSGGLGSEPSRAIRNSRYLKAAQELLDEVVNVWNSIKQKAQKEQVEAGKTEGKENEGGGPKSEGPQESGANAAPELSTAEKQELQNKMAKLMAMLDEVDRKYKHYYHQMQSVVSSFDMVAGAGAAKPYTAVALQTISRHFRCLKDAINDQISVIRKKLGEEESSSGREGRLTRLRYIDQQLRQQRAFQQYGMIPQNAWRPQRGLPENSVTILRAWLFEHFLHPYPKDSEKLMLARQTGLTRSQISNWFINARVRLWKPMIEDMYKEETGDIEQDSNSSSDNAPAGSKAKTASSRDKEDHLARSCCTPRAVCESSSQLSEPPGGASMRAMSVAAAAAGAPMGLFQNEPGHDHDHDDSFMSLMLKEQRPNDGGGGLLLHHSAVAQHQDENARFMAYHLAELGRYGNGNVSLTLGLQRSSSSLSVPHAQQSFPGVGIGVGDDDIYNATAAPLGVSVASPDYESMSQMDQRQRFEQSPLLHDFVA
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGCTACCTACTACTCGAGCCAGGGCAGTGAAAGGGAGCCGTCGCAGAACATGTACTCCAGAGACCCCGGCAATGGCACCGCGTCCTACCCTCCTCTGCCGTCGGCTCTGGGGAACTTGCTCTACTTGAACAGCCCGGCTTCTGGCGGGCCGTACACGGATTTCGGCGGCGTCCAGCAGGACTGCATCGAGATGCCTGAACCTGGACATCCTTCGGTGATGTCCCATGACTCGTCTGCCAGGGAGATCGAGCCCGACATGCTGGGGCAGCAGCGGCGCTCGTTCGGCCTAGCCAGAGGCATGAAGGACGAGGTGCTGATGCGCATGATGGATGGATCAGGATCACATAGCAGCGCAGCCGATGCCATCCATGACGACGCCCCGAATGGCATACAGCTCGATTTCGGCGTTCTGGGCAGCCACGGCCCGCCGGCGCAGGGCGGCCAAGGCTCCTTGTCTCTGAGCCTCAACACGCACATCATGGCGCCTGCCTTGCCGTACTGGCCTGTCGTCAAGGCAGACATGCTGTCGCCGCACTCCTACGACCATGGCTGCGGCGGCGGCAGCGGCGGCCTTGGCTCGGAGCCGTCGCGGGCGATCCGGAACTCGAGGTATCTGAAGGCGGCACAGGAGCTGCTTGACGAGGTCGTCAACGTCTGGAACAGCATCAAGCAGAAGGCCCAGAAAGAGCAGGTGGAGGCAGGGAAGACGGAAGGGAAGGAGAACGAGGGAGGAGGGCCCAAGAGTGAGGGTCCGCAGGAGTCTGGCGCCAACGCTGCACCGGAGCTTTCTACTGCCGAGAAGCAGGAGCTTCAGAACAAGATGGCGAAGCTGATGGCGATGCTGGACGAGGTGGACCGGAAGTACAAGCACTACTACCACCAAATGCAGAGCGTGGTCTCGTCCTTCGACATGGTAGCGGGGGCTGGAGCCGCCAAGCCATACACCGCGGTGGCTCTGCAGACGATCTCGCGCCACTTCCGGTGCCTCAAGGACGCCATCAACGACCAGATCAGCGTCATCAGGAAGAAGCTCGGGGAGGAGGAGAGCTCGTCCGGCAGGGAGGGCAGGCTGACCCGTCTCCGCTACATCGACCAGCAGCTCAGGCAGCAGCGGGCGTTCCAGCAGTACGGCATGATCCCGCAGAACGCTTGGCGGCCACAGAGGGGCCTTCCTGAGAACTCCGTCACCATTCTTCGTGCTTGGCTCTTCGAGCACTTCCTTCACCCGTACCCTAAGGATTCTGAAAAGCTGATGCTGGCAAGGCAAACAGGCCTGACGAGGAGTCAGATTTCGAACTGGTTCATCAACGCCCGCGTCCGCCTCTGGAAACCCATGATCGAGGACATGTACAAGGAAGAGACCGGAGACATCGAGCAAGACTCCAACTCCTCCTCCGACAACGCGCCAGCAGGAAGCAAGGCCAAGACGGCGTCTTCGCGAGACAAGGAGGACCACCTGGCGAGAAGCTGCTGCACGCCGCGGGCCGTCTGCGAGAGCAGCAGCCAGCTGAGCGAGCCACCAGGAGGAGCCAGCATGAGGGCCATGAGCGTCGCCGCCGCCGCCGCTGGAGCGCCCATGGGGTTATTCCAGAACGAGCCTGGCCACGACCACGACCACGACGACAGCTTCATGAGCCTGATGCTCAAGGAGCAGCGACCGAACGACGGCGGCGGCGGCCTCCTCCTCCACCACAGCGCCGTGGCGCAGCACCAGGACGAGAACGCCCGGTTCATGGCGTACCACCTGGCGGAGCTGGGCAGGTACGGCAACGGCAACGTGTCGCTCACCCTGGGGCTGCAGCGCTCCAGCAGCAGCCTCTCGGTTCCGCACGCTCAGCAGAGCTTCCCCGGTGTCGGAATCGGTGTCGGAGACGACGACATCTACAATGCCACCGCCGCTCCTCTCGGTGTCAGCGTGGCGTCGCCGGACTACGAGTCCATGAGCCAGATGGATCAGCGGCAACGGTTCGAGCAGTCGCCCCTTCTCCATGATTTTGTGGCG
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