| TFome pUT5755 | |
|---|---|
| Template | BT086399.1 |
| Genbank Insert ID | KJ727803 |
| Protein Name | ZmALF10 |
| Species | Zea mays |
| TF Family | Alfin-like |
| Gene id | GRMZM2G050495 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
| Subgenome: | 2 |
TFome clone pUT5755
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | B409_F |
| Temperature (ºC) | 73.7 |
| Sequence
|
caccATGGCGGACGCCGCCTCT |
3' Primer
| Name | B409_R |
| Temperature (ºC) | |
| Sequence
|
CTGCCTCGCCCTCTTGGAGCTGCAG |
PCR Condition
5 l GC buffer (NEB) 1 l primer forward 20mM 1 l pr
Amino Acid Sequence
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MDRHGSVRLPTPEDVFTDFRARRDGILKALTTDVEEFYKLCDPEYETLCLYGLPNETWKVNVRPAKVPQQLPEPAEGINIPRDSMPKEDWLSFVAAHSDAWLVAMAFHFGALFGFDKDARRRLHMMINNHPTVSEVVIGSGEKQPKACNTNYETKSSSIKEPSSSSKLAEEPLPKKERQIIKEDGGGEDEAYPCGTCGGMYSENGVFWIGCDICDKWYHGDCVRITPAEATHIDQYSCPACSNKRSRE
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGACAGGCACGGCTCGGTTCGGCTGCCCACCCCCGAGGATGTCTTCACGGATTTCCGCGCCCGCCGTGACGGCATTCTCAAGGCACTCACCACCGATGTGGAGGAGTTCTACAAGTTGTGCGACCCAGAGTATGAGACCCTGTGCCTGTATGGACTACCTAACGAAACCTGGAAAGTGAACGTTCGTCCTGCGAAAGTTCCTCAACAACTACCAGAGCCAGCAGAGGGAATAAACATTCCTCGCGATTCAATGCCAAAGGAAGATTGGCTCTCGTTCGTAGCTGCACACAGTGATGCATGGTTGGTTGCTATGGCATTTCACTTTGGGGCTCTCTTCGGATTTGATAAAGACGCCAGGAGACGGCTCCACATGATGATCAATAATCATCCCACTGTATCTGAAGTTGTGATAGGGAGTGGGGAGAAGCAACCAAAAGCCTGCAACACAAATTACGAAACCAAGTCAAGTTCTATTAAGGAACCCAGTTCTAGCAGCAAGCTAGCAGAGGAGCCTTTGCCAAAGAAGGAGCGACAGATCATCAAAGAAGATGGAGGCGGCGAGGATGAAGCTTACCCATGCGGCACATGTGGAGGGATGTACTCGGAAAATGGAGTATTCTGGATTGGCTGTGACATTTGCGACAAGTGGTACCATGGTGACTGTGTTCGCATCACACCTGCGGAGGCAACGCACATCGATCAGTACAGTTGCCCCGCTTGCAGCAACAAGAGGAGCAGAGAG
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