| TFome pUT5893 | |
|---|---|
| Template | RT-PCR |
| Genbank Insert ID | KJ727849 |
| Protein Name | ZmNAC84 |
| Species | Zea mays |
| TF Family | NAC |
| Gene id | GRMZM2G139700 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | c.T439C p.Y147H Not in functional domain. |
| Request Information | Deposited to ABRC |
| Subgenome: | 2 |
TFome clone pUT5893
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | R103F |
| Temperature (ºC) | 67.5 |
| Sequence
|
caccATGGAGAGGTTGGGCGTCGGC |
3' Primer
| Name | R103R |
| Temperature (ºC) | |
| Sequence
|
GAAGCCCCAGGCGAAGGCG |
PCR Condition
Amino Acid Sequence
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MERLGVGVGVGELPPGFRFHPTDEELITYYLLCKAVDGGFCGGRAIAEIDLNKCEPWELPDKAKMGEKEWYFYCLRDRKYPTGLRTNRATAAGYWKATGKDREVRSGRSGALVGMKKTLVFYRGRAPRGQKTRWVMHEYRLDGTYAHHFLPGSTRDEWVIARVFQKPGEVPCGRKHRLGGPSAAAGESCFSDSTTSASIGGGGGGGASASSRPLLTVTDTSSPSLFVANANAAASNNNGNPVTGRELVPCFSTTASPLEAAALGVVGHPYNAAPLRLGLDFEAPSPGFVVPNLRSLQVQDDGGLPLFLSAAAGGGMSSATLGIMGSLGGSLHCPPHAGMDVVKVEGRAAPPQMAVGPGLLDGAFAWGF
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGAGAGGTTGGGCGTCGGCGTCGGCGTCGGCGAGCTGCCGCCGGGGTTCCGCTTCCACCCGACGGACGAGGAGCTGATCACCTACTACCTCCTCTGCAAGGCCGTGGACGGCGGCTTCTGCGGCGGCCGCGCCATCGCGGAGATCGACCTGAACAAGTGCGAGCCATGGGAGCTCCCGGACAAGGCGAAGATGGGGGAGAAGGAGTGGTACTTCTACTGCCTCCGCGACCGCAAGTACCCGACGGGCCTGCGCACCAACCGCGCCACGGCGGCCGGCTACTGGAAGGCCACCGGCAAGGACCGCGAGGTCCGCAGCGGCCGCAGCGGCGCGCTGGTGGGCATGAAGAAGACGCTCGTCTTCTACCGGGGCCGCGCCCCCAGGGGCCAGAAGACGCGCTGGGTCATGCACGAGTACCGCCTCGACGGCACCTACGCCCACCATTTCCTTCCCGGCTCTACGAGGGACGAGTGGGTGATCGCGAGGGTGTTCCAGAAGCCAGGCGAGGTCCCATGCGGCCGCAAGCACCGCCTGGGCGGCCCCAGCGCCGCCGCCGGCGAGTCCTGCTTCTCGGACTCCACCACCTCGGCCTCCATCGGCGGCGGCGGCGGAGGAGGAGCGTCCGCGTCGTCTCGCCCGCTGCTCACCGTCACGGACACTTCCTCGCCGTCGCTGTTCGTGGCCAACGCGAACGCCGCCGCCAGCAACAACAACGGCAACCCGGTCACCGGGCGAGAGCTCGTGCCCTGCTTCTCCACTACCGCCAGTCCCCTGGAAGCCGCGGCGCTCGGCGTCGTCGGGCACCCGTACAACGCGGCCCCGCTGCGTCTGGGCTTGGACTTCGAGGCGCCGTCCCCGGGCTTCGTCGTCCCGAACCTGCGTTCCCTGCAAGTGCAGGACGACGGCGGCCTGCCGCTGTTCCTCTCGGCAGCAGCAGGCGGCGGCATGTCGTCCGCGACGCTGGGAATAATGGGGTCGCTGGGCGGGTCTCTCCACTGCCCGCCCCACGCCGGCATGGATGTCGTCAAGGTCGAGGGCCGCGCCGCGCCGCCGCAGATGGCTGTCGGCCCCGGCCTCCTCGATGGCGCCTTCGCCTGGGGCTTC
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