| TFome pUT5952 | |
|---|---|
| Template | BT041970.1 |
| Genbank Insert ID | KJ727860 |
| Protein Name | ZmGRAS49 |
| Species | Zea mays |
| TF Family | GRAS |
| Gene id | GRMZM5G821439 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
TFome clone pUT5952
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | B611F |
| Temperature (ºC) | 68.2 |
| Sequence
|
caccATGGACTTGCACCAGTTGTTAAAGTAC |
3' Primer
| Name | B611R |
| Temperature (ºC) | |
| Sequence
|
GTGCCAAGCAGAAGCAGATATCAAC |
PCR Condition
ddH2O----------------11.0 GC buffer-------------5.
Amino Acid Sequence
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MDLHQLLKYRLTGANVYYEFPTENNLANTPWPASPLKSEFSSSSYTPLSSQLECDNLSALSNTPDNQSSTETISAQSISPLEVDSSYKQAAILRENTQVRPDPLYTTSRHNMQHALREIETVLMAPDADDAATSTKHEFEEHKPAQLMRQRSRTWSHESRQPSPGVVRTQLASGYPTASYEFRPEKRLRELREDPQSMVKQLLTKCAEALSEERIEEFLNLVQQARGVVSITGEPIQRLGAYLLEGLVARHANSGTNIYRALKCREPESNELLSYMKILYNICPYLKFGYMAANGAIAEALRNEDRIHIIDFQIAQGTQWITLIQALAARPGGPPHVRITGIDDPVSEYARGEGLDLVGKMLKSMSEEFRIPLEFTPLPGIYATQVTKEMLDIRSGEALAVNFTLQLHHTPDESVDVNNPRDGLLRMVKGLSPKVTTLVEQESHTNTTPFLMRFTETLDYYSAMFESIDTNLPRDNKERINVEQHCLAKDIVNIIACEGKDRVERHELLGKWRSRLTMAGFRPYPLSSYVNSVIRKLLACYSDKYTLEEKDGAMLLGWKNRKLISASAWH
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGACTTGCACCAGTTGTTAAAGTACAGATTAACTGGTGCTAACGTCTACTACGAATTTCCTACAGAGAACAACTTAGCAAACACTCCTTGGCCAGCTAGTCCACTGAAGTCTGAGTTCAGCAGCTCATCATACACTCCTCTTTCATCCCAGCTTGAGTGTGATAATTTGTCTGCACTCAGCAATACTCCAGATAACCAGAGCTCCACAGAAACCATTTCAGCACAATCAATATCACCACTGGAAGTAGACAGCTCATACAAACAGGCAGCTATCCTTCGGGAGAACACCCAAGTGAGACCTGATCCATTGTACACAACATCAAGGCATAATATGCAACACGCTTTACGAGAAATAGAGACTGTTCTGATGGCACCTGATGCTGATGATGCAGCTACTAGCACCAAGCATGAATTTGAGGAACATAAACCTGCTCAGCTGATGAGGCAGCGGTCAAGGACATGGAGTCATGAATCTAGGCAGCCATCACCAGGAGTTGTACGGACACAACTTGCATCTGGATACCCCACAGCAAGCTATGAGTTCCGCCCAGAGAAACGGCTAAGGGAGTTAAGGGAGGATCCGCAGAGCATGGTGAAGCAGCTATTAACCAAGTGTGCTGAAGCATTAAGTGAGGAGAGGATAGAGGAGTTCCTTAACCTTGTTCAGCAAGCTCGTGGAGTTGTATCAATTACTGGGGAGCCAATCCAGCGGTTAGGTGCTTACCTCCTTGAGGGTTTGGTTGCTAGACATGCAAACTCAGGTACAAACATATATCGTGCCCTGAAGTGCCGTGAGCCAGAGAGTAATGAACTTTTGTCTTACATGAAGATTTTGTATAACATCTGTCCGTACTTGAAGTTTGGCTATATGGCTGCCAATGGGGCAATTGCAGAGGCATTAAGAAATGAGGACAGGATCCATATTATTGATTTCCAGATTGCCCAAGGGACACAGTGGATAACACTGATACAAGCGCTAGCTGCAAGGCCTGGGGGTCCACCACACGTGCGGATCACTGGAATAGATGACCCAGTGTCAGAGTATGCCCGTGGTGAAGGTCTGGACCTTGTAGGGAAGATGTTGAAAAGCATGTCTGAGGAATTCAGGATACCTTTGGAGTTTACCCCTCTGCCCGGTATTTATGCAACTCAAGTTACAAAAGAAATGCTGGACATTAGGTCAGGCGAAGCACTTGCTGTCAACTTCACTCTGCAGCTGCACCACACCCCGGATGAGAGCGTGGACGTGAACAACCCACGGGACGGGCTACTCCGGATGGTGAAAGGCCTGTCTCCGAAGGTGACCACGCTTGTGGAGCAGGAGTCGCACACCAACACGACCCCGTTCTTGATGCGGTTCACCGAGACCTTGGACTACTATTCGGCCATGTTCGAGTCAATCGACACGAACCTGCCACGGGACAACAAGGAGCGGATCAACGTGGAGCAGCACTGTCTTGCCAAGGACATCGTGAACATCATCGCCTGCGAGGGGAAGGACCGGGTGGAGCGTCACGAGCTCCTGGGCAAATGGCGGTCGAGGCTGACCATGGCCGGGTTCAGACCATACCCGCTGAGCTCGTACGTGAACTCGGTGATCAGGAAGCTCCTTGCCTGCTACTCGGACAAGTACACCCTGGAAGAGAAGGATGGCGCGATGCTGCTCGGCTGGAAGAACAGGAAGTTGATATCTGCTTCTGCTTGGCAC
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