| TFome pUT5987 | |
|---|---|
| Template | BT067193 |
| Genbank Insert ID | KJ727894 |
| Protein Name | ZmOrphan42 |
| Species | Zea mays |
| TF Family | Orphans |
| Gene id | AC208201.3_FG002 |
| transcript | T002 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
| Subgenome: | 1 |
TFome clone pUT5987
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | B936_F |
| Temperature (ºC) | 71.6 |
| Sequence
|
caccATGTCTTGCACGGTGGCCATCC |
3' Primer
| Name | B936_R |
| Temperature (ºC) | |
| Sequence
|
CGAGCTTGAAAATTTCTGCAACTGC |
PCR Condition
PCR Conditions: Volumes per reaction (25l): 5 l GC
Amino Acid Sequence
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MSCTVAIPSSPVFSPSRRPLSCKAASASPEPTVPASTAAPAPAAAAGSPLRPFALRALLREEVSPSPPPQPTSAAAVALVPSGAALKRRRPTPLVVPASGAAAAAAAAAAVAAVEADPRNEVEEEGEEFAAYCRRGKGRRRVEMEDRHVAKVALGGDPQVALFGVFDGHGGKNAAEFAAENMPKFMAEELTKVNGGEIEGAVKRGYLKTDEEFLKRDESGGACCVTAVLQKGGLVVSNAGDCRAVLSRAGKAEALTSDHRASREDEKERIENLGGFVVNYRGTWRVQGSLAVSRGIGDGHLKQWVVADPDTTTLLVDQQCEFLILASDGLWDKIDNQEAVDLARPLCINNDKTSRMAACRMLTETSISRGSTDDISVVIVQLQKFSSS
TFome clones do not contain stop codon at 3'end.
CDS
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ATGTCTTGCACGGTGGCCATCCCGAGCTCGCCAGTCTTCTCGCCCTCACGCCGCCCACTCTCCTGCAAGGCCGCCTCCGCCTCACCGGAGCCCACCGTCCCCGCCTCTACCGCGGCGCCCGCGCCCGCCGCCGCCGCCGGCTCGCCGCTCCGCCCCTTCGCGCTCCGCGCGCTGCTGCGGGAGGAGGTCTCCCCGTCTCCGCCGCCCCAGCCGACCTCCGCCGCAGCGGTGGCTTTGGTACCGAGCGGGGCCGCGCTGAAGCGGCGGCGACCGACGCCGCTTGTCGTGCCGGCGTCCGGCGCCGCGGCAGCTGCTGCTGCGGCGGCGGCGGTGGCCGCTGTGGAGGCGGATCCGAGGAACGAGGTGGAGGAGGAAGGGGAGGAGTTCGCAGCGTACTGCCGGAGGGGAAAGGGAAGGAGAAGGGTGGAAATGGAGGACCGGCATGTGGCCAAGGTCGCTCTCGGCGGGGACCCCCAAGTGGCACTGTTTGGTGTGTTCGATGGCCACGGCGGGAAAAACGCGGCAGAGTTCGCCGCGGAGAACATGCCCAAGTTTATGGCCGAGGAGTTGACGAAGGTAAACGGCGGAGAGATCGAAGGAGCGGTGAAGAGGGGTTACCTCAAGACGGACGAGGAGTTCCTCAAGAGGGACGAGAGCGGGGGCGCGTGCTGCGTCACAGCCGTCCTCCAAAAGGGTGGACTGGTCGTCTCCAACGCTGGAGACTGCCGTGCGGTGCTCAGCCGAGCAGGGAAGGCAGAGGCGCTCACCTCTGACCACCGGGCGTCCAGGGAGGACGAGAAGGAAAGGATTGAGAATTTGGGTGGATTTGTGGTGAATTACCGTGGAACATGGCGAGTCCAGGGCTCCCTGGCAGTGTCTAGGGGCATTGGGGATGGACACCTAAAGCAGTGGGTTGTGGCTGACCCAGACACCACGACGCTCCTGGTTGACCAGCAGTGCGAGTTCTTGATACTTGCTTCTGATGGCCTCTGGGATAAGATCGACAATCAGGAGGCGGTAGACCTTGCACGACCTCTCTGCATCAACAATGACAAGACCTCTCGCATGGCTGCCTGCAGGATGCTCACCGAGACATCGATTTCCAGGGGCTCGACTGATGATATCAGTGTCGTGATCGTGCAGTTGCAGAAATTTTCAAGCTCG
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