| TFome pUT5991 | |
|---|---|
| Template | RT-PCR |
| Genbank Insert ID | KJ727897 |
| Protein Name | ZmPHD3 |
| Species | Zea mays |
| TF Family | PHD |
| Gene id | GRMZM2G097726 |
| transcript | T02 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
| Subgenome: | 1 |
TFome clone pUT5991
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | CD0174_F |
| Temperature (ºC) | 78.8 |
| Sequence
|
caccATGGCGGGGAAGTCGCGACCGCCCA |
3' Primer
| Name | CD0174_R |
| Temperature (ºC) | |
| Sequence
|
CCTTCGCCGCCTTTTTGACTCCACC |
PCR Condition
PCR Conditions: Volumes per reaction (30l): 6 l GC
Amino Acid Sequence
Copy to clipboard
MAGKSRPPKRILESYTIKGSDRVIKPGDCVLMRASDASKPPYVARVEAIEAAGSRGTNVRVRVRWYYRPEESIGGRRPFHGSKEVFLSDHYDVQSADTIEGKCNVHSFRSYTKLDSVNAEDFFCRFEYKSATGSFVPDRIAVFCKCEMPYNPDDLMIQCEECSDWFHPACIGMTIKEAKKLEHFFCQTCTAENGKMVENSHEATAQSEEKPVESKRRRR
TFome clones do not contain stop codon at 3'end.
CDS
Copy to clipboard
ATGGCGGGGAAGTCGCGACCGCCCAAGCGGATCCTCGAGTCCTATACCATCAAGGGTTCGGACAGAGTCATCAAGCCTGGCGATTGCGTGCTAATGAGGGCATCTGATGCTTCAAAGCCACCTTATGTGGCAAGAGTTGAGGCAATTGAAGCAGCAGGATCTCGAGGCACCAATGTGAGAGTACGAGTGCGGTGGTATTATCGACCAGAGGAGTCCATAGGTGGTCGGCGGCCATTTCATGGTTCCAAGGAGGTTTTCCTCTCTGACCACTATGATGTACAAAGTGCTGACACCATTGAGGGGAAATGCAATGTCCACAGCTTCCGTAGCTATACAAAGCTTGATTCTGTCAATGCTGAGGATTTCTTCTGCCGCTTCGAGTATAAATCAGCTACTGGCAGCTTTGTACCAGATCGTATAGCAGTGTTTTGCAAGTGCGAGATGCCATACAATCCAGATGACCTTATGATCCAATGCGAGGAATGTTCTGACTGGTTTCACCCTGCTTGCATTGGGATGACAATCAAAGAAGCAAAGAAACTCGAGCATTTTTTCTGCCAGACCTGTACAGCTGAAAATGGTAAGATGGTTGAGAATTCACATGAAGCTACAGCGCAATCAGAAGAAAAGCCGGTGGAGTCAAAAAGGCGGCGAAGG
Copyright © 2023 Grassius.org | Last updated: 2023-06-26