TFome clone pUT6015

This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.

5' Primer

Name	B462_F
Temperature (ºC)	68.6
Sequence Copy to clipboard	caccATGGCGATGCCACCGAA

3' Primer

Name	B462_R
Temperature (ºC)
Sequence Copy to clipboard	GAAGGTGGTGTTGCTTTCACCCG

PCR Condition

PCR Conditions: Volumes per reaction (25l): 5 l GC

Amino Acid Sequence

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MAMPPKPGDPPQRSPARSPNLNLPCPLPPVPGGAPPPPQQQPGAGLPPPRVGHHRRARSEVAFRFPDDLPGGGGGGFDEIGSEDDLFSTFMDMDKIAGADRDRAAETSSPPRPAKHRHSVSFDGFGMGAAAGGPGGQQDGGGGMFGEVMEAKKAMSSEQLSELAAIDPKRAKRIIANRQSAARSKERKARYITELERKVQTLQTEATTLSAQLTLFQRDTTGLSAENAELKIRLQAMEQQAQLRDALNDALRQEVERLKIATGEMSKSNNEHFNMGMQHVSYSPSFFQLSEQHTVQQHGNIQLPHHFQQPPLNVPSHQMLSHPNSLSNMVQQDSLGRLQGLDIGKGSMAVKSEAEVVVKSEGSSISAGESNTTF

TFome clones do not contain stop codon at 3'end.

CDS

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ATGGCGATGCCACCGAAGCCCGGCGACCCGCCGCAGCGCTCGCCGGCACGTAGCCCAAACCTCAATCTCCCCTGCCCGCTGCCGCCGGTCCCGGGCGGGGCGCCGCCACCGCCGCAGCAGCAGCCGGGCGCCGGGCTGCCCCCGCCGCGCGTGGGCCACCACCGCCGCGCCAGATCTGAGGTGGCCTTCCGCTTCCCGGACGACCTGCCCGGCGGCGGGGGCGGCGGCTTCGACGAGATCGGCTCCGAGGACGACCTCTTCTCCACCTTCATGGACATGGACAAGATCGCCGGCGCCGACCGCGACCGCGCCGCCGAGACCTCCTCGCCGCCGCGCCCAGCCAAGCACCGCCACAGCGTCTCCTTTGACGGCTTCGGGATGGGGGCTGCCGCCGGCGGCCCGGGCGGCCAGCAGGACGGCGGTGGAGGCATGTTCGGAGAGGTGATGGAGGCCAAGAAGGCCATGTCTTCCGAGCAGCTCTCCGAGCTCGCCGCCATCGATCCCAAGCGCGCCAAAAGAATTATTGCAAACAGGCAATCTGCAGCACGGTCAAAGGAAAGAAAAGCTCGATATATAACAGAATTGGAGCGGAAGGTTCAAACTCTTCAGACAGAGGCTACTACTCTTTCAGCACAGCTGACACTATTCCAGAGAGACACAACTGGACTTTCAGCAGAAAATGCTGAGCTCAAGATAAGGTTGCAGGCCATGGAGCAGCAGGCCCAACTGCGTGACGCTCTAAATGATGCACTGAGGCAAGAAGTGGAGAGGCTTAAGATAGCAACGGGCGAGATGTCGAAGTCCAATAACGAGCATTTCAATATGGGAATGCAGCACGTTTCCTACAGCCCTTCATTCTTCCAGCTCTCGGAGCAACACACAGTGCAGCAGCATGGAAACATTCAGCTGCCGCACCATTTCCAACAGCCTCCGCTGAATGTCCCGAGCCACCAGATGCTATCCCACCCCAACTCCCTCTCGAACATGGTGCAGCAAGACTCTCTTGGGCGTCTCCAGGGGCTGGACATTGGGAAAGGCTCCATGGCTGTGAAGTCGGAGGCTGAGGTCGTGGTGAAGTCAGAGGGCAGCTCTATATCTGCGGGTGAAAGCAACACCACCTTC