TFome clone pUT6030

This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.

5' Primer

Name	C523_F
Temperature (ºC)	68.4
Sequence Copy to clipboard	caccATGAGAGGTTTAAGTCGGTATCTGCC

3' Primer

Name	C523_R
Temperature (ºC)
Sequence Copy to clipboard	ACGACGCAGGACGGGCTTC

PCR Condition

PCR Conditions: Volumes per reaction (25l): 5 l GC

Amino Acid Sequence

Copy to clipboard

MRGLSRYLPFCGGGCCCGDGAGRAGGVADGLVWDVALKAHASGDYSIAVAQANEALEDQAQVVAAPAATLVGVFDGHGGPEAARFVNRRLFSHIQAFAAENGGLSAEVFQKAFGATEEEFIGLVQKSWPSQPRIVSVGSCCLVGAVDIENGTLYVANLGDSRAVLGRRRGKGRRVVAERLSQDHNVADEGVRREVAEMHPDDSHIVLNSHGVWRIKGIIQVSRSIGDVYLKKPDICRGNPVLQQSICPFPLRRPVMTAVPSITTRELRPGDRFIIFASDGLWEQLSDDAAVDVVASSPRKGVAMRLVRAAQLEAARKKEVKYDKIRTIEKGQRRHFHDDITVVVLFLDKCRGAARSGPEDIDGTYAPVDVFSCSPAAGGDHEDPTKPVLRR

TFome clones do not contain stop codon at 3'end.

CDS

Copy to clipboard

ATGAGAGGTTTAAGTCGGTATCTGCCGTTCTGCGGCGGCGGGTGCTGCTGCGGTGACGGCGCCGGTAGGGCAGGGGGCGTGGCGGATGGGCTGGTGTGGGACGTGGCCCTCAAGGCGCACGCGTCCGGTGACTACTCCATCGCCGTGGCGCAGGCGAACGAGGCGCTCGAGGACCAGGCGCAGGTGGTCGCGGCTCCGGCCGCCACGCTCGTCGGCGTCTTCGACGGGCACGGCGGGCCCGAGGCCGCGCGCTTCGTCAACAGGCGCCTCTTCTCGCATATCCAAGCCTTTGCGGCTGAGAACGGTGGCCTGTCAGCGGAGGTGTTCCAGAAGGCCTTCGGCGCGACGGAGGAGGAGTTCATAGGCCTGGTGCAGAAATCATGGCCGTCGCAGCCGAGGATCGTGTCCGTGGGCTCCTGCTGCCTGGTGGGCGCCGTCGACATCGAGAACGGGACGCTGTACGTGGCCAACCTGGGCGACTCGCGCGCCGTGCTGGGCCGCCGCCGGGGCAAGGGGAGGCGCGTGGTGGCCGAGCGGCTGTCTCAGGACCACAACGTGGCCGACGAGGGCGTGCGGCGGGAGGTGGCCGAGATGCACCCGGACGACTCGCACATCGTGCTCAACAGCCACGGCGTGTGGCGGATCAAGGGGATCATCCAGGTGTCCAGGTCCATCGGCGATGTGTACCTCAAGAAGCCTGACATCTGCAGGGGCAACCCGGTGCTGCAGCAGTCCATCTGCCCGTTCCCGCTGCGCCGCCCCGTCATGACCGCCGTGCCGTCCATCACCACGCGGGAGCTGCGCCCCGGCGACCGCTTCATCATCTTCGCGTCGGACGGGCTGTGGGAGCAGCTCAGCGACGACGCCGCGGTGGACGTCGTGGCGAGCAGCCCGAGGAAGGGCGTGGCCATGCGCCTGGTGCGGGCCGCCCAGCTGGAGGCGGCGCGCAAGAAGGAGGTCAAGTACGACAAGATCCGGACCATCGAGAAGGGCCAGCGCCGGCACTTCCACGACGACATCACCGTCGTCGTGCTCTTCCTCGACAAGTGCAGGGGAGCTGCCAGGTCCGGCCCCGAGGACATCGACGGCACCTACGCGCCGGTGGACGTGTTCTCCTGCAGCCCCGCCGCCGGCGGCGATCACGAGGACCCGACGAAGCCCGTCCTGCGTCGT