| TFome pUT6144 | |
|---|---|
| Template | RT-PCR |
| Genbank Insert ID | KJ728009 |
| Protein Name | ZmSBP10 |
| Species | Zea mays |
| TF Family | SBP |
| Gene id | GRMZM2G111136 |
| transcript | T02 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
| Subgenome: | 1 |
TFome clone pUT6144
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | R135F |
| Temperature (ºC) | 64.0 |
| Sequence
|
caccATGATGATGAACACCAACGCGATGTCG |
3' Primer
| Name | R135R |
| Temperature (ºC) | |
| Sequence
|
GATGAAGTCCACCTCAAAGAAACCGTTGC |
PCR Condition
Amino Acid Sequence
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MMMNTNAMSPTASTDADFSFGPLQPYVGFDVAGMGMPSVVVERSSSLLQHNTSSSSHNNNLGLYDSFDFAAAAGLPFQESGLLPPASLPLAPSPQSMAAMAMPPSSLLLTLPGVPAAADVVYNPFGGGGAGGFLKREDGGPLVDAAGGGGRIGLNLGRRTYFSPADVLAVDRLLTRSRLAGGGVGVGLGMGLGMGVLGLGAAHHQQPPPRCQAEGCKADLSAAKHYHRRHKVCEYHAKASAVAAAGKQQRFCQQCSRFHVLAEFDEAKRSCRKRLTEHNRRRRKPSSAQDEDSSPPPPKKLDTCITNTASYNDDLKTGASNTAAAAISPNGSGVSCLDVMDNGQTSSAAAPTALSLAAPPPPPLHEKDGSLDSMLMQRVLQGRREDDDDEQRRHFITSLVMQTTQQQHSGGGGAGNILSCSSVSDQHNGGGCNGFFEVDFI
TFome clones do not contain stop codon at 3'end.
CDS
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ATGATGATGAACACCAACGCGATGTCGCCGACAGCAAGTACCGACGCCGACTTCTCGTTCGGCCCCTTGCAGCCCTACGTCGGCTTCGACGTCGCCGGCATGGGCATGCCCAGCGTCGTCGTCGAGAGGTCGTCGTCGCTACTGCAGCACAACACCAGCAGCAGCAGCCACAACAACAACCTAGGGCTATACGACAGTTTCGACTTCGCGGCCGCCGCAGGGTTACCGTTCCAAGAATCTGGTCTCCTGCCCCCGGCGAGCCTGCCGCTGGCGCCGTCGCCGCAAAGCATGGCGGCCATGGCAATGCCACCGTCGTCGCTGCTGCTGACGCTCCCAGGCGTGCCTGCGGCGGCGGATGTCGTCTATAACCCCTTTGGTGGCGGCGGCGCGGGAGGGTTCCTGAAGCGGGAGGACGGCGGCCCCCTCGTGGACGCTGCTGGAGGCGGCGGGAGGATCGGGCTGAACCTCGGGCGCCGCACCTACTTCTCCCCCGCGGACGTACTCGCCGTGGACCGGCTGCTGACGCGGTCGCGGCTGGCGGGTGGCGGCGTGGGCGTGGGCCTGGGTATGGGCCTCGGCATGGGCGTGCTGGGGCTCGGCGCCGCCCACCACCAGCAGCCGCCGCCGCGGTGCCAGGCCGAGGGCTGCAAGGCCGACCTCTCCGCCGCCAAGCACTACCACCGCCGCCACAAGGTCTGCGAGTACCACGCCAAGGCCAGCGCCGTCGCCGCCGCCGGCAAGCAGCAGCGTTTCTGCCAGCAGTGCAGCAGGTTTCATGTGCTTGCTGAGTTTGATGAGGCCAAGAGGAGCTGCCGGAAGCGGCTCACCGAGCACAACCGCCGCCGCCGGAAGCCGAGCAGCGCACAGGACGAGGACTCGTCACCGCCACCACCCAAGAAACTGGATACTTGCATAACTAATACTGCCTCTTACAATGATGATCTCAAGACAGGCGCGAGCAACACGGCAGCTGCGGCCATCTCGCCGAACGGCAGCGGCGTCAGCTGCCTAGACGTCATGGACAACGGCCAGACGAGCAGCGCAGCGGCGCCGACGGCTCTGTCTTTGGCCGCCCCGCCGCCGCCGCCGCTGCACGAGAAGGACGGCAGTCTCGACTCGATGCTGATGCAGCGGGTCCTGCAGGGCCGACGGGAGGACGACGACGACGAACAGCGTCGCCACTTCATCACCTCGCTTGTGATGCAGACGACCCAGCAGCAGCACAGCGGCGGCGGCGGCGCCGGCAACATCTTGTCGTGCTCGTCGGTATCAGATCAGCACAACGGCGGCGGCTGCAACGGTTTCTTTGAGGTGGACTTCATC
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