| TFome pUT6197 | |
|---|---|
| Template | RT-PCR |
| Genbank Insert ID | KJ728051 |
| Protein Name | ZmGATA29 |
| Species | Zea mays |
| TF Family | C2C2-GATA |
| Gene id | GRMZM2G039586 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | c.C941T p.A314V; Not in functional domain |
| Request Information | Deposited to ABRC |
| Subgenome: | 1 |
TFome clone pUT6197
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | R184F |
| Temperature (ºC) | 65.3 |
| Sequence
|
caccATGATCGCTACCTCGCTTTCCCTCTTGA |
3' Primer
| Name | R184R |
| Temperature (ºC) | |
| Sequence
|
GCTCCGGACAAGCCCGCAGGAC |
PCR Condition
Amino Acid Sequence
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MIATSLSLLIPGCKLPFRHNNHCTSRFGSPLPTLHISHPDLHSSPNHTRSAYIAPSDLERKFLSVHPMSAIYMSQLSTALPLMEGDHQDHHHHHQGHFQAFTLPKETPILFPFVISNSSASEGSLSYGSAADHHLLRQRHQAMLEPQHMIGGSSTATGNSVFSTPFPTVESIRDDMIEPASYDPYDMGKLQVGGSMDACSWTPAAAKMRITRKATADPSAGKKPRRRAQAGYDDTMSGQPNLGVIRVCSDCNTTKTPLWRSGPCGPKSLCNACGIRQRKARRAMMAAASGSVSAVPTDSGKASPSNAAVAAAAVHPKVKKEKRVDVDRSLPFKKRCKVVQQGHGAAVVVAAPAAATDSATVVQATAEDGDDDTCPSRDLLVDDIGGLISWSRSPAAPASAAAATCSFRASPALPVQQDEITDAAMLLMTLSCGLVRS
TFome clones do not contain stop codon at 3'end.
CDS
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ATGATCGCTACCTCGCTTTCCCTCTTGATCCCTGGCTGCAAATTGCCATTCCGACATAATAACCACTGCACCAGCCGCTTCGGTTCGCCTCTTCCTACTCTCCATATATCTCATCCTGATCTTCACTCATCACCTAACCATACGAGATCTGCATATATAGCTCCATCAGATCTTGAGCGCAAATTCCTCTCAGTGCATCCCATGTCTGCCATCTACATGAGCCAGCTCTCCACTGCCCTCCCTCTCATGGAGGGAGATCACCAAGATCATCATCACCACCACCAAGGCCACTTCCAGGCCTTCACCCTGCCAAAGGAAACACCGATCCTTTTCCCCTTTGTGATCAGCAATAGTAGCGCCAGCGAAGGCAGTTTGAGCTATGGATCGGCTGCAGACCATCACTTGTTGAGGCAGCGTCATCAAGCTATGCTGGAGCCCCAACATATGATTGGAGGATCATCAACTGCGACTGGGAATAGTGTCTTCTCGACGCCGTTCCCGACCGTGGAGAGCATCCGCGACGACATGATCGAGCCTGCCTCGTACGATCCATACGATATGGGGAAGCTGCAGGTCGGCGGGTCGATGGATGCTTGCAGCTGGACGCCGGCGGCAGCCAAGATGAGGATCACGAGGAAGGCCACTGCCGATCCCAGTGCGGGGAAGAAGCCAAGGAGAAGGGCGCAAGCAGGGTACGACGACACCATGAGCGGCCAACCGAATTTGGGTGTTATTAGGGTGTGCTCCGACTGCAACACCACCAAGACACCCTTGTGGAGGAGTGGTCCTTGCGGCCCCAAGTCGCTCTGCAACGCGTGCGGTATCAGGCAGCGGAAGGCGAGGCGGGCGATGATGGCCGCCGCCTCCGGCTCGGTGTCAGCAGTTCCCACTGACAGCGGCAAGGCCTCGCCCAGCAACGCCGCCGTGGCCGCAGCAGCAGTACACCCCAAGGTGAAGAAGGAGAAGAGAGTGGACGTGGACCGGTCGCTGCCGTTCAAGAAACGGTGCAAGGTGGTCCAGCAAGGTCACGGTGCGGCCGTCGTCGTTGCAGCTCCTGCTGCTGCCACCGACAGCGCGACTGTCGTGCAGGCCACCGCCGAGGATGGTGATGACGACACCTGTCCGAGCAGGGATCTGCTTGTCGACGACATTGGTGGGCTCATCAGCTGGAGCAGGAGTCCGGCGGCACCCGCCTCAGCGGCTGCGGCCACCTGCAGCTTCCGGGCGTCGCCGGCGTTGCCCGTGCAGCAGGACGAGATCACGGACGCTGCCATGCTGCTGATGACGCTGTCCTGCGGGCTTGTCCGGAGC
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