| TFome pUT6477 | |
|---|---|
| Template | BT064296 |
| Genbank Insert ID | KJ728201 |
| Protein Name | ZmOrphan214 |
| Species | Zea mays |
| TF Family | Orphans |
| Gene id | GRMZM2G052699 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
| Subgenome: | 1 |
TFome clone pUT6477
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | C528_F |
| Temperature (ºC) | 71.3 |
| Sequence
|
caccATGTCTTCGCGCTCGGGGTC |
3' Primer
| Name | C528_R |
| Temperature (ºC) | |
| Sequence
|
CCCACTGTTTCTTGATGAATCTCG |
PCR Condition
PCR Conditions: Volumes per reaction (25l): 5 l GC
Amino Acid Sequence
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MSSRSGSTGGGSTGGGSSAGGGGAVPLAVLLRREVASERTASERPELHWGLFSQAKKGEDFTFLKPECERVPDVPSSSFSAFGLFDGHNGNGAAIYTKENLLNNILSAVPADLNREDWLAALPRALVAAFVKTDKDFQTKARSSGTTVTFVIIDGLVITVASVGDSRCVLQAEGSIYHLSSDHRFDASKEEVDRVTECGGDVGRLNVVGGAEIGPLRCWPGGLCLSRSIGDQDVGEFIVPVPLVKQVKLSTAGGRLIIASDGVWDVLSPEVAFNCSRELPPVPAAEQIVKTAVQSKGLRDDTTCIVVDIIVEKNNSSMPLAKRQPGIGVFKNMFGKKKSSDSSSHSHSDREYMDPDNVEEIFEDGCALLSRRLDSEYPVRNMFKLFICAICQVELKPNQGISVHEDSSQPGSLRRWDGPFLCQSCQEKKEAMEGKRRSRDSSRNSG
TFome clones do not contain stop codon at 3'end.
CDS
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ATGTCTTCGCGCTCGGGGTCCACGGGCGGAGGGAGCACCGGCGGCGGCAGCAGCGCGGGGGGAGGCGGCGCGGTGCCGCTGGCCGTGCTGCTCAGGCGGGAGGTGGCCAGCGAGCGCACCGCGTCGGAGCGCCCGGAGCTGCACTGGGGCCTCTTCAGCCAGGCCAAGAAGGGCGAGGACTTCACCTTCCTCAAGCCCGAATGCGAGCGCGTCCCTGACGTCCCGTCCTCCTCCTTCTCCGCCTTCGGCCTGTTTGATGGGCATAATGGGAACGGAGCCGCAATTTACACCAAGGAGAATCTCTTGAACAACATCTTGAGTGCGGTCCCAGCCGATCTGAACAGGGAAGACTGGCTTGCTGCACTCCCAAGAGCGCTGGTTGCTGCATTCGTCAAAACCGATAAGGATTTCCAGACTAAAGCACGTTCTTCAGGGACAACTGTGACATTTGTCATAATAGATGGATTGGTCATCACTGTTGCATCAGTTGGTGATTCCCGTTGTGTACTACAAGCTGAAGGTTCAATTTATCATCTATCCTCTGATCATCGGTTCGATGCAAGCAAAGAGGAGGTTGATCGTGTAACAGAATGCGGAGGTGATGTTGGAAGGCTAAATGTTGTTGGTGGTGCTGAGATTGGCCCCCTTAGATGTTGGCCTGGAGGTTTGTGCCTATCAAGGTCAATTGGAGATCAAGATGTTGGTGAATTTATCGTTCCAGTTCCTCTTGTGAAGCAAGTTAAGCTATCTACTGCGGGTGGCCGACTCATTATTGCAAGTGATGGTGTTTGGGATGTCTTGTCTCCAGAAGTAGCTTTTAACTGTTCACGTGAACTTCCTCCAGTGCCTGCAGCTGAGCAAATCGTTAAAACTGCTGTGCAATCAAAAGGGCTGAGGGATGATACCACCTGTATTGTTGTTGATATAATAGTAGAAAAAAACAACTCTTCCATGCCACTTGCTAAAAGGCAACCAGGAATTGGTGTCTTCAAAAATATGTTCGGCAAGAAAAAATCATCGGATTCATCATCCCATTCCCATTCAGATAGAGAATATATGGATCCAGATAACGTGGAGGAAATATTTGAGGATGGGTGTGCGCTTCTTTCTAGACGGCTGGATTCTGAATACCCTGTTCGAAATATGTTCAAACTGTTCATATGTGCCATTTGTCAAGTAGAGTTAAAGCCAAACCAAGGTATATCTGTACATGAAGATTCATCACAACCTGGGAGCTTGCGTCGCTGGGATGGTCCATTCCTTTGCCAGAGCTGTCAGGAAAAGAAAGAAGCAATGGAGGGAAAGCGTCGTTCACGAGATTCATCAAGAAACAGTGGG
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