| TFome pUT6499 | |
|---|---|
| Template | BT061588 |
| Genbank Insert ID | KJ728222 |
| Protein Name | ZmOrphan249 |
| Species | Zea mays |
| TF Family | Orphans |
| Gene id | GRMZM2G177386 |
| transcript | T03 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
TFome clone pUT6499
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | B923_F |
| Temperature (ºC) | 71.2 |
| Sequence
|
caccATGAGGCTCGCACTGAAGAAGGGTA |
3' Primer
| Name | B923_R |
| Temperature (ºC) | |
| Sequence
|
TGAGTTGCTCTTGGCCTTCTTTCG |
PCR Condition
10 l GC buffer (Thermo Fisher) 2 l primer forward
Amino Acid Sequence
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MRLALKKGTEDNITVIVVDLKPRKKAKSNS
TFome clones do not contain stop codon at 3'end.
CDS
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ATGAGGCTCGCACTGAAGAAGGGTACCGAGGACAATATCACTGTCATTGTGGTTGACTTGAAACCTCGAAAGAAGGCCAAGAGCAACTCA
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