| TFome pUT6503 | |
|---|---|
| Template | BT065915 |
| Genbank Insert ID | KJ728226 |
| Protein Name | ZmOrphan226 |
| Species | Zea mays |
| TF Family | Orphans |
| Gene id | GRMZM5G836628 |
| transcript | T02 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
TFome clone pUT6503
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | C500_F |
| Temperature (ºC) | 74.1 |
| Sequence
|
caccATGGCGACCGCCGCGAGG |
3' Primer
| Name | C500_R |
| Temperature (ºC) | |
| Sequence
|
CCTGCATGCTGTAGAGCGGCTA |
PCR Condition
PCR Conditions: Volumes per reaction (25l): 5 l GC
Amino Acid Sequence
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MATAARERRLPPALPLATLIGRELRAGGSERPALRYGHAGFAKRGEDYFLVKPDCLRVPGDPASAFSVFAVFDGHNGVSAAVYSKEHLLEHVMSALSPDIGREDWLQALPRALVAGFVKADIDFQRKGEVSGTTATLVVIDGFTVTVASVGDSRCILDTQGGELQLLTVDHRLEENAEERERVTASGGEVGRLNLFGGQEVGPLRCWPGGLCLSRSIGDMDVGEFIVPIPHVKQVKLSNTGGRLIIASDGIWDALSNEAAAKACRGLPAELAAKLVVKQALKTSGLKDDTTCVVVDIIPSDHLTSPQLSPKKNQNKLKSLFRRRSHSSVRKLGGKSASIGSVEELFEEGSAMLEERLGRNLSLKAASPPFRCAICQVDQEPFQDLMADNGGGYCSSQYAPWGGPCLCLDCRKKKDAMEGKRCSRSTACR
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGCGACCGCCGCGAGGGAGAGGCGCCTGCCGCCGGCGCTGCCCCTGGCGACTCTCATCGGCCGGGAGCTCCGCGCGGGGGGCTCCGAGCGCCCGGCCCTGCGCTACGGCCACGCCGGCTTTGCCAAGCGTGGCGAGGACTACTTCCTCGTCAAGCCCGACTGCCTCCGCGTCCCCGGGGACCCCGCATCCGCGTTCTCCGTCTTTGCCGTGTTCGACGGCCACAACGGCGTGTCGGCGGCGGTCTACAGCAAGGAGCATCTGCTCGAGCACGTCATGAGCGCGCTGTCGCCGGACATCGGCCGCGAAGACTGGCTGCAGGCGCTGCCGCGCGCGCTCGTCGCCGGCTTCGTCAAGGCCGACATCGATTTCCAGCGCAAGGGGGAGGTGTCCGGAACCACGGCGACACTGGTGGTCATCGATGGGTTCACGGTCACGGTGGCGTCGGTGGGTGACTCGCGCTGCATCCTGGACACGCAGGGCGGCGAGTTGCAGCTGCTAACCGTGGACCACCGCCTGGAGGAGAATGCAGAGGAGCGGGAGCGCGTCACGGCCAGCGGTGGGGAAGTAGGCCGGCTCAATCTCTTTGGCGGCCAGGAGGTCGGCCCTCTCCGGTGCTGGCCTGGTGGCTTGTGCCTCTCAAGATCCATCGGGGACATGGATGTTGGAGAGTTCATTGTGCCCATTCCACATGTCAAGCAAGTCAAGCTATCAAACACCGGAGGAAGACTGATAATAGCATCTGATGGCATATGGGACGCGTTGTCCAATGAAGCAGCTGCAAAAGCATGCAGAGGATTGCCTGCAGAACTGGCAGCGAAGCTTGTCGTTAAGCAAGCTCTGAAAACAAGCGGGCTAAAGGATGACACCACCTGTGTCGTAGTTGACATTATCCCGTCCGATCATCTGACATCGCCACAATTGTCTCCAAAGAAAAATCAGAACAAGTTGAAGTCACTTTTTCGTAGAAGGTCGCATAGTTCAGTTAGAAAGCTTGGAGGCAAGTCTGCCTCCATTGGTTCCGTGGAGGAGTTATTTGAAGAAGGGTCTGCAATGCTAGAAGAAAGGTTGGGTAGGAATCTGTCCTTGAAAGCAGCTTCACCACCTTTCCGCTGTGCAATCTGCCAAGTGGACCAAGAGCCCTTCCAAGATTTGATGGCAGATAATGGAGGTGGTTACTGCTCTTCCCAGTACGCTCCATGGGGTGGCCCATGTCTCTGTTTGGACTGTCGGAAAAAGAAAGATGCAATGGAAGGAAAAAGATGTAGCCGCTCTACAGCATGCAGG
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