| TFome pUT6544 | |
|---|---|
| Template | Genomic DNA |
| Genbank Insert ID | KJ728267 |
| Protein Name | ZmLBD15 |
| Species | Zea mays |
| TF Family | LBD |
| Gene id | AC207888.3_FG009 |
| transcript | T009 |
| Vector | pENTR/SD/D-TOPO |
| Note | c.C401G p.A134G Not in functional domain |
| Request Information | Deposited to ABRC |
TFome clone pUT6544
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | SE131_F |
| Temperature (ºC) | 77.6 |
| Sequence
|
caccATGCCGGCGGACGGGGGCG |
3' Primer
| Name | SE131_R |
| Temperature (ºC) | |
| Sequence
|
CAATCCTACATATAATGGGAATGGGAATGGGAATGGCGCG |
PCR Condition
Step 1, 98°C 3 min melt, 1 cycle; Step 2, 98&d
Amino Acid Sequence
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MPADGGAVAGAPCGACRTLRCRCVPRCVFAAYFSAEEFAAVHGVFGASNVSKMPEDIGLPEQRRLAVDTLVEEARAWARDPTFDRVSYLCILQDQEVNDKYREQVDDAREEFDAEVASEPVDVAAAGLEAQAQGQVDAALQHEQDYRLLGGWGATTGEQQHLVTQDAENEHGIILGHGHPAELSQEILQLQELLDMAELSRNRKQQIIQKTEMGQYWDAAAGGAREHDLLTMMQQAPAAAWQHQYPAPQYAGIRGGPGGHQEMPQPMEEACTAEFATGMGIMSQQFASAQPYHDHDPAAVPHASPWLQRMPEAQQPAAASQVASEQDMLLLLLLQQHQVAAAGLMSAGGARQLDNVAAQYDDTEVALGYGYGHPDMHQHQHQQVAAAGQVAGVDDDMNPREMTQQQHAQQGLDIALGNGHLDGETEAMLQELAALLEQAGKQETIRKQQGVATGMGQWDAVTGIAWEQDVTTMTHQQSPDAAAWLQVQEMLEQMEEASSTPEFAEIIAWQAEARYAQEELAITLGNDHPDWSTQDILQAQELFAMAELARKQEMVRNLVAAAALAREQEMIAQQQQHPEAQYSGTELDISLWQGPGHGHPYWHQPTMQQMLQEKQLPTVQQLLQEQQLADAIGFGTHQASTTLVPQYANGEHGSGTGAAMAFQSPGSDKDAPFPFPFPLYVGL
TFome clones do not contain stop codon at 3'end.
CDS
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ATGCCGGCGGACGGGGGCGCTGTGGCGGGGGCGCCATGCGGGGCGTGCAGGACGCTCCGGTGCAGATGCGTGCCGCGCTGCGTCTTCGCGGCCTACTTCTCGGCGGAGGAGTTCGCCGCCGTGCACGGCGTCTTCGGCGCCAGCAACGTGTCCAAGATGCCCGAGGACATCGGACTCCCCGAGCAGAGGCGGCTCGCCGTGGACACGCTCGTCGAAGAGGCCAGGGCGTGGGCGCGGGACCCCACCTTCGACCGCGTCTCCTACCTCTGCATCCTCCAGGACCAGGAGGTGAACGACAAGTACAGGGAGCAGGTCGACGATGCGCGGGAGGAGTTCGACGCCGAGGTCGCGTCCGAGCCGGTCGACGTTGCTGCCGCAGGGCTGGAGGCGCAGGCGCAGGGGCAGGTCGACGCCGCGCTGCAGCACGAGCAGGACTACAGGCTGCTGGGCGGTTGGGGGGCGACGACGGGCGAGCAGCAGCACCTTGTGACGCAAGACGCCGAGAATGAGCACGGCATCATCCTTGGGCACGGCCACCCGGCCGAGCTATCCCAGGAGATTCTGCAGCTGCAGGAGTTGTTGGATATGGCAGAGCTCTCCAGAAACAGAAAGCAGCAGATCATCCAGAAAACAGAGATGGGGCAGTACTGGGACGCGGCGGCGGGGGGCGCGAGGGAACATGACCTACTGACGATGATGCAGCAGGCCCCGGCCGCTGCGTGGCAGCACCAATACCCCGCCCCGCAGTATGCCGGGATTCGTGGCGGGCCTGGCGGCCACCAGGAGATGCCGCAGCCGATGGAGGAAGCCTGCACGGCGGAGTTTGCTACGGGGATGGGCATCATGTCGCAGCAATTCGCCTCGGCGCAGCCGTACCACGACCACGACCCTGCGGCGGTGCCCCACGCGTCGCCATGGCTCCAGCGTATGCCTGAGGCGCAGCAGCCGGCTGCCGCGTCGCAGGTGGCGAGCGAGCAGGACATGCTGCTGTTGCTGCTACTGCAGCAGCATCAGGTCGCGGCGGCGGGGTTGATGTCCGCGGGCGGCGCTCGTCAGTTGGATAACGTCGCCGCACAATACGATGACACGGAGGTCGCACTGGGGTACGGGTACGGCCATCCGGACATGCACCAGCACCAGCACCAGCAGGTGGCCGCCGCGGGGCAGGTTGCCGGAGTGGATGACGACATGAACCCCCGGGAGATGACGCAGCAGCAGCACGCTCAGCAAGGGCTCGACATCGCCCTTGGAAACGGCCACCTGGACGGCGAAACCGAGGCGATGCTGCAGGAGTTGGCCGCCCTGCTAGAGCAGGCGGGAAAGCAGGAAACGATCAGGAAGCAGCAGGGCGTGGCGACAGGGATGGGGCAGTGGGACGCGGTGACGGGGATCGCGTGGGAGCAGGACGTGACGACGATGACGCACCAGCAGAGCCCGGACGCTGCTGCGTGGCTCCAGGTCCAGGAGATGCTGGAGCAGATGGAGGAAGCGTCGTCCACGCCGGAGTTTGCTGAGATCATAGCGTGGCAAGCCGAGGCGCGGTACGCCCAGGAAGAGCTCGCCATCACCCTTGGTAACGACCACCCGGACTGGTCAACCCAGGATATCCTGCAAGCGCAGGAGTTGTTCGCCATGGCAGAGCTGGCCAGGAAGCAGGAAATGGTCAGGAATCTGGTCGCCGCGGCGGCGCTCGCCAGAGAGCAGGAAATGATCGCGCAGCAGCAGCAGCACCCCGAGGCGCAGTACTCCGGGACAGAGCTCGACATCTCGCTGTGGCAAGGCCCCGGCCACGGCCACCCGTACTGGCATCAGCCGACGATGCAGCAGATGCTGCAGGAGAAGCAGCTGCCGACGGTGCAACAGCTGTTGCAGGAGCAGCAGCTGGCCGACGCGATTGGATTCGGCACGCACCAGGCCAGCACGACTTTGGTGCCGCAGTATGCGAACGGTGAGCACGGGAGCGGCACCGGCGCCGCAATGGCGTTCCAGTCGCCGGGGTCTGACAAGGACGCGCCATTCCCATTCCCATTCCCATTATATGTAGGATTG
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