TFome clone pUT6566

This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.

5' Primer

Name	B948_F
Temperature (ºC)	68.3
Sequence Copy to clipboard	caccATGATGAGCTTCAAGGGCCAC

3' Primer

Name	B948_R
Temperature (ºC)
Sequence Copy to clipboard	CGCCTTGAGGAGCTCGC

PCR Condition

5 l GC buffer (Thermo Fisher) 1 l primer forward 2

Amino Acid Sequence

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MMSFKGHEGFGQVAAAGAGSQAASHGGAGPLPWWAGPQLLFGEPAPPSPEETRRDAQFQVVPGVQGTPDPAPPKTGTPEVLKFSVFQGNLESGGKGEKTPKNSTTIALQSPFPEYNGRFEIGLGQSMLAPSNYPCADQCYGMLAAYGMRSMSGGRMLLPLNATADAPIYVNPKQYEGILRRRRARAKAESENRLAKGRKPYLHESRHLHAMRRVRGTGGRFVNTKKEGRGTGVASNGGSKTAAAAPSRLAMPPSFQSSVASLSGSDVSNMYSGGLEQHLRAPHFFTPLPPIMEDGDHGGPPTRISSSFKWAASDGCCELLKA

TFome clones do not contain stop codon at 3'end.

CDS

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ATGATGAGCTTCAAGGGCCACGAGGGGTTTGGCCAGGTGGCCGCCGCCGGTGCCGGGAGCCAGGCTGCCTCCCATGGTGGAGCAGGCCCGCTGCCATGGTGGGCGGGGCCCCAGCTGCTGTTCGGCGAGCCGGCGCCCCCGTCACCGGAGGAGACGCGCCGGGACGCCCAGTTCCAGGTCGTGCCGGGGGTTCAGGGCACGCCGGATCCAGCGCCGCCCAAGACAGGGACACCTGAGGTCCTCAAATTCTCTGTCTTTCAAGGGAATTTGGAGTCGGGTGGTAAAGGAGAGAAAACCCCAAAGAACTCTACCACTATTGCTCTTCAGTCACCGTTCCCAGAATACAATGGCCGTTTCGAGATTGGTCTTGGTCAATCTATGCTGGCCCCTTCCAATTATCCTTGTGCTGACCAGTGCTATGGCATGCTTGCGGCTTATGGAATGAGATCGATGTCTGGTGGGAGAATGCTGTTGCCACTAAATGCGACAGCTGATGCACCCATCTATGTGAATCCGAAGCAGTACGAAGGCATCCTCCGCCGTCGCCGTGCTCGCGCCAAGGCGGAGAGCGAGAACAGGCTCGCCAAAGGCAGAAAGCCCTATCTCCACGAGTCGCGCCACCTCCACGCGATGCGTCGGGTAAGAGGCACCGGCGGGCGCTTCGTCAACACGAAGAAAGAAGGGCGTGGCACGGGCGTTGCTTCGAACGGGGGCAGCAAGACGGCTGCAGCGGCACCGTCGCGCCTCGCCATGCCCCCTAGCTTCCAGAGTAGCGTCGCCAGCCTGTCTGGCTCCGACGTGTCAAACATGTACAGCGGCGGCTTGGAGCAGCACCTTCGGGCGCCGCACTTCTTCACCCCGCTGCCACCCATCATGGAGGACGGCGACCACGGTGGTCCCCCCACCCGCATCTCCTCCTCCTTCAAGTGGGCAGCCAGCGACGGCTGCTGCGAGCTCCTCAAGGCG