| TFome pUT6609 | |
|---|---|
| Template | BT067450.1 |
| Genbank Insert ID | KJ728328 |
| Protein Name | ZmPHD36 |
| Species | Zea mays |
| TF Family | PHD |
| Gene id | GRMZM5G889372 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | c.G288T p.Q96H c.G1716Tp.E572D c.G2284T p.A762S. E572D present in Ring finger domain. None present in template FlcDNA. |
| Request Information | Deposited to ABRC |
TFome clone pUT6609
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | C326_F |
| Temperature (ºC) | 70.9 |
| Sequence
|
caccATGGGCACTGGAGAGGGGT |
3' Primer
| Name | C326_R |
| Temperature (ºC) | |
| Sequence
|
CTGCATGTTCCTGAGGTTGCTCTTG |
PCR Condition
Volumes per reaction (25l): 5 l GC buffer (Thermo
Amino Acid Sequence
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MGTGEGSPPARRGISGRRRRWPDEDDDEYVLEEEEEEDYVEELSASSAGEEGVDSDAEYQEAEEEDEIEKPRPKRPAKGITSSAGERGLDSDAEYHEAEEDDEIEPPRLKRPAKGSTSSAGEEGLDSDAEYQEAEEDEMETPRPKRPAKDSTSSAGEDGLDSDAEYQEAEEEDDDEIETPRPKRRAKGSNRVRKGKVDLAAARSRRRKYEEDEDYEEEVDEDDEVEEEYHDDLEEVEEEEEVAPQRPKSIVKCGARGRNVKLSLAAKRSHQRKQEDDEDMDFDPELDDEEVEIDEDIDFDPDVDGDEEDEYQDEEEEELVPARARKVTVKNPVKRKSISKRRTPKKKKKNRGSKVSRRKGASAKVKKSAPVRRRRKRSVIDDYEDEDVDDDDDDDFIVDDEVIVNRHPKKKARIGSGRETVVDPQVSIEEETWPDLESDSSDFEFATSDEEPNNVETPVAEQTTVRKGRKKRISESESSSDSEFVVSGKELEELREPEPPKAAPNLHVSLRRICITRHGEGKGKEKQEQEEAGKPICGICLSEELRATVQGVLNCCSHYFCFACIMEWSRVDSRCPLCKQRFTTITKSSKVDLGLGVRKAVVKVEERDQVYQPTEEEIRRWLDPYENVVCIECNQGGDDNLMLLCDICDSSAHTYCVGLGREVPEGNWYCGGCRLGDEGPSYTGIQRTVAYERQNYRSHVGSSVSFGMAVPGGTFERPSSINPRHSFQGIDLNLSPREFPGESHPAESQVSTDSVSTPTGRSGRTISGRRELQRYIQSLFRPSLPSRPDTYHNIAQNSGGVSRNEPNRWNFPSSSEANTSQNLPDGIQNHHSDLPFVQAHSNFAPFMSPDGDDFQQIEGVKSNLRNMQ
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGGCACTGGAGAGGGGTCGCCGCCGGCGAGGCGGGGCATCAGCGGTAGGCGCCGTCGATGGCCGGACGAGGACGACGACGAGTATGTGCTGGAGGAGGAAGAAGAAGAGGATTATGTGGAGGAGCTCTCCGCCTCGAGCGCCGGCGAGGAGGGGGTGGATTCAGACGCCGAGTACCAGGAGGCAGAGGAGGAGGACGAGATCGAGAAGCCGCGCCCAAAGCGACCAGCGAAGGGCATCACATCGAGCGCCGGCGAGAGGGGATTGGATTCAGACGCCGAATACCATGAGGCAGAGGAGGACGACGAGATCGAGCCGCCGCGCCTGAAGCGACCGGCCAAGGGCAGCACATCGAGCGCGGGTGAGGAGGGGTTGGATTCAGACGCCGAATACCAGGAGGCAGAGGAGGACGAGATGGAGACGCCGCGCCCAAAGCGACCGGCGAAGGACAGCACATCGAGCGCCGGCGAGGATGGGTTGGATTCAGACGCCGAATACCAGGAGGCAGAGGAGGAGGACGACGACGAAATCGAGACGCCGCGCCCGAAGCGACGGGCAAAGGGCAGCAACAGAGTACGGAAAGGAAAGGTGGATCTGGCCGCCGCACGATCTCGACGCCGGAAGTATGAGGAGGACGAGGACTACGAGGAGGAGGTTGACGAAGACGACGAGGTGGAGGAGGAGTACCATGATGACCTTGAGGAGGTCGAGGAGGAGGAAGAAGTGGCACCACAGCGGCCGAAGAGCATTGTGAAATGCGGTGCCCGTGGCCGGAATGTGAAGCTGTCTCTGGCAGCGAAGCGATCTCATCAGCGGAAGCAGGAGGATGATGAGGATATGGATTTTGACCCAGAGTTGGATGACGAGGAGGTCGAGATAGATGAGGATATTGATTTTGATCCCGATGTCGATGGTGACGAGGAGGATGAGTATCAAGACGAGGAGGAAGAGGAACTGGTTCCTGCCCGTGCCAGAAAGGTAACTGTAAAGAATCCGGTGAAGCGAAAGTCCATTTCAAAGAGGCGAACACCAAAGAAGAAGAAGAAAAATAGGGGATCTAAGGTTTCTAGGCGGAAGGGGGCTTCAGCTAAAGTGAAGAAATCAGCACCTGTTAGGAGGCGGAGAAAGCGTTCAGTGATAGATGATTACGAGGATGAGGATGTGGATGATGACGATGACGACGACTTCATTGTCGACGATGAGGTCATTGTGAATCGGCACCCCAAGAAGAAGGCCAGGATAGGGAGTGGGAGGGAGACAGTGGTGGACCCTCAAGTATCTATTGAGGAAGAGACATGGCCAGACCTTGAGTCAGACTCATCGGACTTTGAGTTTGCGACGTCCGATGAAGAACCTAACAATGTTGAAACACCTGTAGCTGAGCAGACTACAGTCAGAAAAGGGAGGAAGAAGAGGATATCTGAGTCAGAGTCATCCTCAGATTCTGAATTTGTTGTATCAGGTAAGGAATTGGAAGAATTGAGGGAGCCTGAGCCTCCAAAGGCTGCACCAAATCTGCATGTGTCCTTGAGAAGGATTTGTATTACAAGGCATGGAGAAGGCAAGGGAAAGGAGAAACAGGAACAGGAGGAGGCTGGGAAGCCAATATGCGGGATCTGTCTCTCGGAGGAGCTGAGAGCTACTGTACAAGGTGTCCTCAATTGTTGCTCACACTATTTTTGCTTCGCATGCATCATGGAATGGTCTAGGGTTGATTCGAGATGCCCATTGTGCAAGCAGCGGTTCACTACAATCACCAAGTCATCAAAGGTAGATCTTGGTCTTGGAGTGAGAAAAGCTGTAGTCAAGGTCGAAGAGCGTGATCAGGTTTATCAGCCCACTGAAGAAGAAATAAGGCGTTGGCTAGACCCATATGAAAATGTTGTTTGTATAGAGTGCAATCAAGGTGGCGATGATAATCTCATGCTACTATGTGACATTTGTGATTCCTCAGCACATACTTATTGTGTTGGCTTGGGTAGAGAAGTGCCCGAAGGAAACTGGTACTGTGGAGGATGTAGACTCGGTGACGAGGGACCATCATATACTGGAATTCAGCGTACAGTAGCTTATGAAAGACAAAATTACAGAAGTCATGTCGGTAGTTCTGTAAGTTTTGGCATGGCTGTACCAGGCGGTACATTTGAAAGGCCGTCATCAATCAATCCTCGCCATTCTTTTCAAGGGATTGATCTAAATCTATCTCCAAGAGAGTTTCCCGGAGAATCACATCCTGCTGAATCACAAGTTTCAACTGATAGCGTATCAACTCCCACTGGAAGATCTGGAAGAACTATTTCAGGAAGGCGTGAATTACAACGGTACATTCAGAGTTTGTTCAGACCAAGTCTACCTAGTAGACCAGACACGTACCACAATATTGCGCAGAACAGTGGTGGTGTATCAAGAAATGAACCGAACCGCTGGAACTTCCCTTCTTCATCAGAAGCAAACACATCACAGAATCTGCCTGATGGCATTCAGAACCATCACAGTGACCTTCCATTTGTTCAAGCTCACAGCAATTTTGCTCCATTTATGTCCCCAGATGGGGATGATTTCCAGCAAATTGAGGGTGTCAAGAGCAACCTCAGGAACATGCAG
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