| TFome pUT6622 | |
|---|---|
| Template | BT038195 |
| Genbank Insert ID | KJ728341 |
| Protein Name | ZmALF19 |
| Species | Zea mays |
| TF Family | Alfin-like |
| Gene id | GRMZM2G047316 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
TFome clone pUT6622
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | B408_F |
| Temperature (ºC) | 73.1 |
| Sequence
|
caccATGGACGGCGGCGGCAC |
3' Primer
| Name | B408_R |
| Temperature (ºC) | |
| Sequence
|
TGCTCGGGCTCTCTTGCCACTACC |
PCR Condition
PCR Conditions: Volumes per reaction (25l): 5 l GC
Amino Acid Sequence
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MAGPVSSAPRTVEDIYKDYAARRAGLVRALTSDVDGFYSMCDPEKENLCLYGLPNGGWEVSLPVEEVPPEMPEPALGINFARDGMRRRDWLSLVAVHSDAWLVSVAFFFAAKLNGNDRKRLFNMINDHPSVYEIMADRKGRENNPGVDNSSKSRHSTKRSNDGKIKNSRVAVGECRYENDEDHSETLCGSCSGLYNSSEFWIGCDICERWFHGKCVRITPAKAEQIKHYKCPDCSYKKSRQ
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGCCGGCCCCGTCTCCTCAGCGCCCCGGACCGTCGAGGACATCTACAAGGACTACGCCGCCCGCCGCGCCGGCCTCGTCCGCGCTCTCACATCTGATGTCGACGGGTTCTACAGCATGTGCGACCCAGAGAAGGAGAACCTGTGCCTGTACGGTCTCCCGAACGGCGGCTGGGAGGTATCGCTGCCGGTGGAGGAGGTGCCGCCGGAGATGCCCGAACCAGCGCTCGGGATCAACTTCGCCAGGGACGGCATGAGGCGTCGCGACTGGCTCTCGCTCGTCGCCGTACACTCTGACGCTTGGCTCGTCTCCGTCGCATTCTTCTTCGCCGCCAAACTCAACGGCAACGACAGGAAGCGATTATTCAACATGATTAATGATCATCCATCTGTGTATGAAATAATGGCTGACCGGAAGGGAAGGGAGAATAATCCTGGTGTTGATAACAGCAGCAAATCCAGGCACTCAACGAAGCGATCAAATGATGGAAAGATAAAGAACTCAAGAGTGGCAGTTGGTGAATGTAGGTATGAGAATGATGAAGACCACAGCGAAACCCTTTGTGGTTCTTGCAGTGGTCTCTACAACTCAAGTGAGTTCTGGATTGGATGCGACATTTGTGAGCGGTGGTTCCACGGCAAATGTGTGAGGATAACCCCAGCAAAAGCAGAACAGATAAAGCACTACAAGTGCCCTGATTGCAGCTACAAAAAGAGCAGGCAA
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