TFome clone pUT6642

This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.

5' Primer

Name	CD0154_F
Temperature (ºC)	67.4
Sequence Copy to clipboard	caccATGTCGTCCTTGCTATGTATTAGCC

3' Primer

Name	CD0154_R
Temperature (ºC)
Sequence Copy to clipboard	GGAGGAGGAGTCGAGCATCTTCTTG

PCR Condition

PCR Conditions: Volumes per reaction (30l): 6 l GC

Amino Acid Sequence

Copy to clipboard

MSSLLCISRRNNKKDSDGDGEYGGEQIIISTGGAAMRKGPWTDEEDEQLVRFVRLFGERRWDFLAKVSGLRRTGKSCRLRWVNYLHPGLRRGRITADEERRIVELHAQWGSRWSRIARSLPGRTDNEIKNFWRTRTRKKALEERRAAAAATSTAAESASSVMTVSSSSECRRPPVSSSSSSSSLRRDEARRSGDETEEASTPTAAPATKHDEEPQPQPQECYDFPMDQLWNEIAAADAAAMSYLVVDACWGGAAVGGHCYYYGAAAASAASPPPPASPPWEYCGSDFSLWRIDDQEYYKKMLDSSS

TFome clones do not contain stop codon at 3'end.

CDS

Copy to clipboard

ATGTCGTCCTTGCTATGTATTAGCCGCAGGAACAACAAGAAGGACAGCGATGGCGATGGAGAGTACGGTGGCGAGCAGATCATCATCAGCACGGGCGGCGCGGCCATGAGGAAGGGCCCGTGGACGGACGAGGAGGACGAGCAGCTGGTACGGTTCGTGCGCCTGTTCGGCGAACGCCGATGGGATTTCTTAGCAAAGGTGTCAGGTCTGAGGCGCACCGGCAAGAGCTGCCGCCTCCGGTGGGTGAACTACCTCCACCCGGGGCTCCGGCGCGGCCGCATCACGGCCGATGAGGAGCGCCGCATCGTGGAGCTCCACGCGCAGTGGGGCAGCCGGTGGTCGCGCATCGCCAGGAGCCTGCCCGGGCGCACCGACAACGAGATCAAGAACTTCTGGAGGACGCGGACCAGGAAGAAGGCGCTCGAGGAGAGGAGGGCTGCCGCAGCAGCAACGTCGACGGCGGCGGAGTCGGCGTCCTCGGTGATGACGGTGTCGTCGTCGTCCGAGTGCCGCCGCCCGCCCGTATCGTCGTCGTCGTCGTCGTCTTCGCTACGGCGGGACGAAGCGCGGCGCAGCGGCGACGAGACCGAGGAGGCGTCCACGCCCACCGCGGCGCCGGCGACCAAGCACGATGAGGAACCCCAGCCGCAGCCGCAGGAGTGCTACGACTTCCCCATGGACCAGCTGTGGAACGAGATCGCGGCAGCCGATGCGGCGGCGATGAGCTACCTTGTCGTGGACGCCTGCTGGGGCGGAGCTGCTGTTGGTGGTCACTGTTATTACTACGGCGCCGCCGCAGCCTCAGCCGCCTCGCCGCCGCCGCCGGCCTCGCCGCCGTGGGAGTACTGCGGCTCCGATTTTTCGCTGTGGAGGATCGACGACCAAGAGTACTACAAGAAGATGCTCGACTCCTCCTCC